A triply cloned strain of Xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orange

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dc.contributor.authorLi, W. B.
dc.contributor.authorZreik, L.
dc.contributor.authorFernandes, N. G.
dc.contributor.authorMiranda, V. S.
dc.contributor.authorTeixeira, D. C.
dc.contributor.authorAyres, A. J.
dc.contributor.authorGarnier, M.
dc.contributor.authorBove, J. M.
dc.contributor.institutionINRA
dc.contributor.institutionUniv Bordeaux 2
dc.contributor.institutionFundo Paulista Defesa Citricultura Fundecitrus
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:19:38Z
dc.date.available2014-05-20T15:19:38Z
dc.date.issued1999-08-01
dc.description.abstractXylella fastidiosa isolate 8.1,b obtained from a sweet orange tree affected by citrus variegated chlorosis in the state of Sb Paulo, Brazil, and shown in 1993 to be the causal agent of the disease, was cloned by repeated culture in liquid and on solid PW medium, yielding triply cloned strain 9a5c. The eighth and the 16th passages of strain 9a5c were mechanically inoculated into sweet orange plants. Presence of X. fastidiosa in sweet orange leaves of shoots having grown after inoculation (first-flush shoots) was detected by DAS-ELISA and PCR. Thirty-eight days after inoculation, 70% of the 20 inoculated plants rested positive, and all plants gave strong positive reactions 90 days after inoculation. Symptoms first appeared after 3 months and were conspicuous after 5 months. X. fastidiosa was reisolated from sweet orange leaves, 44 days after inoculation. These results indicate that X. fastidiosa strain 9a5c, derived from pathogenic isolate 8.1.b by triply cloning, is also pathogenic, Strain 9a5c is now used for the X. fastidiosa genome sequencing project undertaken on a large scale in Brazil.en
dc.description.affiliationINRA, Biol Cellulaire & Mol Lab, F-33883 Villenave Dornon, France
dc.description.affiliationUniv Bordeaux 2, F-33883 Villenave Dornon, France
dc.description.affiliationFundo Paulista Defesa Citricultura Fundecitrus, BR-14807040 São Paulo, Brazil
dc.description.affiliationUNESP, FCAV, Dept Plant Protect, São Paulo, Brazil
dc.description.affiliationUnespUNESP, FCAV, Dept Plant Protect, São Paulo, Brazil
dc.format.extent106-108
dc.identifierhttp://dx.doi.org/10.1007/s002849900428
dc.identifier.citationCurrent Microbiology. New York: Springer Verlag, v. 39, n. 2, p. 106-108, 1999.
dc.identifier.doi10.1007/s002849900428
dc.identifier.issn0343-8651
dc.identifier.urihttp://hdl.handle.net/11449/31073
dc.identifier.wosWOS:000081486100011
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCurrent Microbiology
dc.relation.ispartofjcr1.373
dc.relation.ispartofsjr0,562
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleA triply cloned strain of Xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orangeen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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