Caracterização bioquímica e molecular de Cryptococcus spp. isolados de excretas ambientais de pombos (Columba livia domestica)

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dc.contributor.authorColombo, G. [UNESP]
dc.contributor.authorTáparo, C. V. [UNESP]
dc.contributor.authorAraújo, E. C. [UNESP]
dc.contributor.authorMakatu, M. Y. [UNESP]
dc.contributor.authorSantos, F. S. [UNESP]
dc.contributor.authorMarinho, M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:40:28Z
dc.date.available2018-12-11T16:40:28Z
dc.date.issued2015-01-01
dc.description.abstractPigeons have a rich diversity of microrganisms, including fungi saprobes such as the genus Cryptococcus that can act as a pathogen for humans and animals. The aim of this was their isolation, biochemical and molecular characterization of samples of Cryptococcus from ambientas avian excreta. One hundred environmental samples,representative of the area equidistant from Araçatuba, São Paulo points were collected. The samples were removed and stored in collection bottles, sent to the Laboratory of Bacteriology and Mycology of FMVA, where they were processed and cultivated in duplicate on Petri dishes containing Sabouraud dextrose 4% and Niger and incubated at room temperature 30° C, respectively, for a period of no less than 15 days. Colonies after daily observation which were suggestive for yeast growth were re-isolated in Niger agar and subjected to biochemical analisis for further molecular characterization by PCR tests. As results, the biochemical and molecular characterization of 32 yeast colonies revealed 8% of cultures positive for Cryptococcus neoformans var. neoformans, 17% Rhodotorula rubidae and 7% Candida albicans. It was concluded that environmental avian excreta are active sites for replication of Cryptococcus neoformans var. neoformans and other yeasts with pathogenic potential. These constitute a public health risk, and preventive measures are necessary, such as cleaning and complete removal of excreta in order to minimize the risk of environmental exposure.en
dc.description.affiliationFaculdade de Medicina Veterinária de Araçatuba FMVA- Unesp
dc.description.affiliationUnespFaculdade de Medicina Veterinária de Araçatuba FMVA- Unesp
dc.format.extent1639-1645
dc.identifierhttp://dx.doi.org/10.1590/1678-4162-7753
dc.identifier.citationArquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 67, n. 6, p. 1639-1645, 2015.
dc.identifier.doi10.1590/1678-4162-7753
dc.identifier.fileS0102-09352015000601639.pdf
dc.identifier.issn1678-4162
dc.identifier.issn0102-0935
dc.identifier.scieloS0102-09352015000601639
dc.identifier.scopus2-s2.0-84952042460
dc.identifier.urihttp://hdl.handle.net/11449/168256
dc.language.isopor
dc.relation.ispartofArquivo Brasileiro de Medicina Veterinaria e Zootecnia
dc.relation.ispartofsjr0,248
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiochemist
dc.subjectCryptococcus sp
dc.subjectPCR
dc.subjectPigeons
dc.subjectPublic health
dc.titleCaracterização bioquímica e molecular de Cryptococcus spp. isolados de excretas ambientais de pombos (Columba livia domestica)pt
dc.title.alternativeBiochemical and molecular characterization of Cryptococcus spp. isolates from environmental feces of pigeons (Columba livia domestic)en
dc.typeArtigo

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