Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury

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dc.contributor.authorSant'Ana, Monielle
dc.contributor.authorSouza, Helena R. [UNESP]
dc.contributor.authorPossebon, Lucas [UNESP]
dc.contributor.authorCornélio, Marinônio L. [UNESP]
dc.contributor.authorRiffo-Vasquez, Yanira
dc.contributor.authorGirol, Ana Paula [UNESP]
dc.contributor.authorOliani, Sonia M. [UNESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversity Center Padre Albino (UNIFIPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionKing's College London
dc.date.accessioned2020-12-12T01:55:13Z
dc.date.available2020-12-12T01:55:13Z
dc.date.issued2020-04-01
dc.description.abstractChronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke.en
dc.description.affiliationDepartment of Morphology and Genetics São Paulo Federal University (UNIFESP)
dc.description.affiliationUniversity Center Padre Albino (UNIFIPA)
dc.description.affiliationDepartment of Physics São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto Campus
dc.description.affiliationSackler Institute of Pulmonary Pharmacology Institute of Pharmaceutical Science King's College London
dc.description.affiliationDepartment of Biology Laboratory of Immunomorphology São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto Campus
dc.description.affiliationUnespDepartment of Physics São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto Campus
dc.description.affiliationUnespDepartment of Biology Laboratory of Immunomorphology São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto Campus
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2012/13041-4
dc.description.sponsorshipIdFAPESP: 2016/02012-4
dc.description.sponsorshipIdCNPq: 308144/2014-7
dc.description.sponsorshipIdCNPq: 404190/2016-2
dc.identifierhttp://dx.doi.org/10.1016/j.pupt.2020.101896
dc.identifier.citationPulmonary Pharmacology and Therapeutics, v. 61.
dc.identifier.doi10.1016/j.pupt.2020.101896
dc.identifier.issn1522-9629
dc.identifier.issn1094-5539
dc.identifier.scopus2-s2.0-85078662244
dc.identifier.urihttp://hdl.handle.net/11449/200010
dc.language.isoeng
dc.relation.ispartofPulmonary Pharmacology and Therapeutics
dc.sourceScopus
dc.subjectAnnexin A1
dc.subjectBronchoalveolar lavage
dc.subjectChronic obstructive pulmonary disease
dc.subjectInflammatory cells
dc.subjectMice
dc.subjectSmoking
dc.titleEffect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injuryen
dc.typeArtigo

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