Immunoexpression of S100A4 in canine skin melanomas and correlation with histopathological parameters

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dc.contributor.authorGrandi, Fabrizio [UNESP]
dc.contributor.authorRocha, Rafael M.
dc.contributor.authorMiot, Hélio Amante [UNESP]
dc.contributor.authorCogliati, Bruno
dc.contributor.authorRocha, Noeme Sousa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundacao Antonio Prudente
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-03-18T15:54:33Z
dc.date.available2015-03-18T15:54:33Z
dc.date.issued2014-01-01
dc.description.abstractBackground: Melanoma is one of the most common skin neoplasms in humans and dogs. The tumor microenvironment in melanoma comprises cancer cells and stromal cells that interact to accelerate tumor progression. Several prognostic markers for melanomas have been studied in many human tumors, including fibroblast-specific protein 1 (S100A4). S100A4 is a member of the S100 family of calcium-binding proteins in stromal cells.Hypothesis/objectives: The objective of this study was to describe the immunohistochemical patterns of S100A4 in stroma and neoplastic cells of canine skin melanomas and correlate them with some histological parameters.Animals and Methods: Forty-eight samples (38 pigmented and 10 non-pigmented melanomas) were first selected and their nature confirmed using S100, Melan A and vimentin. All cases were examined by immunohistochemistry using S100A4 to correlate expression, histotype, and level of invasion.Results: All the tumors, including 10 non-pigmented, were positive for S100, Melan A, vimentin and negative for cytokeratin AE1/AE3 (consistent with melanomas). The 48 melanomas were classified as epithelioid (n = 21), spindle (n = 14), and mixed (n = 13). S100A4 was preferentially expressed in epithelioid and spindle cell types compared with mixed melanomas and S100A4 expression was not associated with level of invasion (Clark's levels IV to V).Conclusion: S100A4 expression in melanoma samples varied among histotypes but not between levels of invasion.en
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Vet Clin, Lab Invest & Comparat Pathol, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Dept Pathol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationFundacao Antonio Prudente, Natl Inst Oncogen, Hosp AC Camargo, Dept Oncol, BR-01509010 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Botucatu Med Sch, Dept Dermatol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508270 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Vet Clin, Lab Invest & Comparat Pathol, Sch Vet Med & Anim Sci, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Dept Pathol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Botucatu Med Sch, Dept Dermatol, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 10/14317-8
dc.description.sponsorshipIdFAPESP: 11/01610-1
dc.format.extent98-104
dc.identifierhttp://dx.doi.org/10.1080/01652176.2014.936628
dc.identifier.citationVeterinary Quarterly. Abingdon: Taylor & Francis Ltd, v. 34, n. 2, p. 98-104, 2014.
dc.identifier.doi10.1080/01652176.2014.936628
dc.identifier.issn0165-2176
dc.identifier.lattes2543633050941005
dc.identifier.lattes6077735918469284
dc.identifier.orcid0000-0002-8188-8149
dc.identifier.urihttp://hdl.handle.net/11449/116941
dc.identifier.wosWOS:000342291700007
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofVeterinary Quarterly
dc.relation.ispartofjcr1.492
dc.relation.ispartofsjr0,520
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdogen
dc.subjectcanineen
dc.subjectmelanomaen
dc.subjectskinen
dc.subjectfibroblast-specific protein 1en
dc.subjectS100A4en
dc.subjectstromaen
dc.subjectimmunohistochemistryen
dc.titleImmunoexpression of S100A4 in canine skin melanomas and correlation with histopathological parametersen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Ltd
unesp.author.lattes2543633050941005
unesp.author.lattes6077735918469284
unesp.author.orcid0000-0002-1388-7240[4]
unesp.author.orcid0000-0002-2596-9294[3]
unesp.author.orcid0000-0002-8188-8149
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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