Effect of curcumin-nanoemulsion associated with photodynamic therapy in breast adenocarcinoma cell line

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dc.contributor.authorMachado, Francielly Cristina [UNESP]
dc.contributor.authorAdum de Matos, Renata Prandini [UNESP]
dc.contributor.authorPrimo, Fernando Lucas [UNESP]
dc.contributor.authorTedesco, Antonio Claudio
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorCalmon, Marília Freitas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T16:21:56Z
dc.date.available2019-10-06T16:21:56Z
dc.date.issued2019-05-01
dc.description.abstractCurcumin, a natural compound has several antineoplastic activities and is a promising natural photosensitizer used in photodynamic therapy. However, its low solubility in physiological medium limit the clinical use of curcumin. This study aimed to analyze the action of curcumin-nanoemulsion, a new and well-designed Drug Delivery System (DDS+) molecule, used as a photosensitizing agent in photodynamic therapy in an in vitro breast cancer model, MCF-7 cells. The empty nanoemulsion fulfils all necessary requirements to be an excellent DDS. Furthermore, the use of curcumin-nanoemulsion in photodynamic therapy resulted in a high phototoxic effect after activation at 440 nm, decreasing to <10% viable tumor cells after two irradiations and increasing the reactive oxygen species (ROS) production. The use of curcumin-nanoemulsion associated with photodynamic therapy resulted in an increase in the levels of caspase 3/7 activity for the studied MCF-7 cell model, indicating that this therapy triggers a cascade of events that lead to cell death, such as cellular apoptosis. In conclusion, curcumin-nanoemulsion proved to be efficient as a photosensitizing agent, had phototoxic effects, significantly decreased the proliferation of MCF-7 cells and stimulating the ROS production in combination with photodynamic therapy, so, this formulation has a great potential for use in treatment of breast cancer.en
dc.description.affiliationUNESP – São Paulo State University – IBILCE – Institute of Biosciences Humanities and Exact Sciences – Department of Biology – Rua Cristóvão Colombo, 2265 – Bairro Jardim Nazareth
dc.description.affiliationUNESP – São Paulo State University– Faculty of Pharmaceutical Sciences of Araraquara – Department of Bioprocess and Biotechnology, Rodovia Araraquara/Jaú, Km 01, s/n, Campos Ville
dc.description.affiliationDepartment of Chemistry Center for Nanotechnology and Tissue Engineering Faculty of Philosophy Sciences and Languages of Ribeirão Preto University of São Paulo – USP
dc.description.affiliationUnespUNESP – São Paulo State University – IBILCE – Institute of Biosciences Humanities and Exact Sciences – Department of Biology – Rua Cristóvão Colombo, 2265 – Bairro Jardim Nazareth
dc.description.affiliationUnespUNESP – São Paulo State University– Faculty of Pharmaceutical Sciences of Araraquara – Department of Bioprocess and Biotechnology, Rodovia Araraquara/Jaú, Km 01, s/n, Campos Ville
dc.description.sponsorshipUniversidade Estadual Paulista
dc.format.extent1882-1890
dc.identifierhttp://dx.doi.org/10.1016/j.bmc.2019.03.044
dc.identifier.citationBioorganic and Medicinal Chemistry, v. 27, n. 9, p. 1882-1890, 2019.
dc.identifier.doi10.1016/j.bmc.2019.03.044
dc.identifier.issn1464-3391
dc.identifier.issn0968-0896
dc.identifier.lattes7991082362671212
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.scopus2-s2.0-85063336990
dc.identifier.urihttp://hdl.handle.net/11449/188873
dc.language.isoeng
dc.relation.ispartofBioorganic and Medicinal Chemistry
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBreast cancer
dc.subjectCurcumin
dc.subjectNanotechnology
dc.subjectPhotodynamic therapy
dc.titleEffect of curcumin-nanoemulsion associated with photodynamic therapy in breast adenocarcinoma cell lineen
dc.typeArtigo
unesp.author.lattes7991082362671212[5]
unesp.author.orcid0000-0001-5693-6148[5]

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