ChIP-seq reveals a role for CrzA in the Aspergillus fumigatus high-osmolarity glycerol response (HOG) signalling pathway

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dc.contributor.authorCastro, Patricia A. de
dc.contributor.authorChen, Chenxi
dc.contributor.authorCouto de Almeida, Ricardo Sergio
dc.contributor.authorFreitas, Fernanda Zanolli [UNESP]
dc.contributor.authorBertolini, Maria Celia [UNESP]
dc.contributor.authorMorais, Enyara Rezende
dc.contributor.authorBrown, Neil Andrew
dc.contributor.authorZambelli Ramalho, Leandra Naira
dc.contributor.authorHagiwara, Daisuke
dc.contributor.authorMitchell, Thomas K.
dc.contributor.authorGoldman, Gustavo H.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionOhio State Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionChiba Univ
dc.contributor.institutionNatl Lab Sci & Technol Bioethanol CTBE
dc.date.accessioned2015-03-18T15:55:20Z
dc.date.available2015-03-18T15:55:20Z
dc.date.issued2014-11-01
dc.description.abstractAspergillus fumigatus is an opportunistic pathogen and allergen of mammals. Calcium signalling is essential for A. fumigatus pathogenicity and is regulated by the CrzA transcription factor. We used ChIP-seq (Chromatin Immunoprecipitation DNA sequencing) to explore CrzA gene targets in A. fumigatus. In total, 165 potential binding peaks including 102 directly regulated genes were identified, resulting in the prediction of the A[GT][CG]CA[AC][AG] CrzA-binding motif. The 102 CrzA putatively regulated genes exhibited a diverse array of functions. The phkB (Afu3g12530) histidine kinase and the sskB (Afu1g10940) MAP kinase kinase kinase of the HOG (high-osmolarity glycerol response) pathway were regulated by CrzA. Several members of the two-component system (TCS) and the HOG pathway were more sensitive to calcium. CrzAen
dc.description.affiliationUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationOhio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Araraquara, Brazil
dc.description.affiliationChiba Univ, Med Mycol Res Ctr, Chiba, Japan
dc.description.affiliationNatl Lab Sci & Technol Bioethanol CTBE, Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Araraquara, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipOhio Agricultural Research and Development Center at the Ohio State University, USA
dc.format.extent655-674
dc.identifierhttp://dx.doi.org/10.1111/mmi.12785
dc.identifier.citationMolecular Microbiology. Hoboken: Wiley-blackwell, v. 94, n. 3, p. 655-674, 2014.
dc.identifier.doi10.1111/mmi.12785
dc.identifier.issn0950-382X
dc.identifier.lattes8817669953838863
dc.identifier.lattes2225250119200162
dc.identifier.orcid0000-0002-8810-2970
dc.identifier.urihttp://hdl.handle.net/11449/117152
dc.identifier.wosWOS:000344465000013
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofMolecular Microbiology
dc.relation.ispartofjcr3.816
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleChIP-seq reveals a role for CrzA in the Aspergillus fumigatus high-osmolarity glycerol response (HOG) signalling pathwayen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes8817669953838863
unesp.author.lattes2225250119200162[4]
unesp.author.orcid0000-0002-8810-2970[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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