Molecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosin

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dc.contributor.authorAoki, Pedro H.B. [UNESP]
dc.contributor.authorMorato, Luis F.C. [UNESP]
dc.contributor.authorPavinatto, Felippe J.
dc.contributor.authorNobre, Thatyane M.
dc.contributor.authorConstantino, Carlos J.L. [UNESP]
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:28:07Z
dc.date.available2018-12-11T17:28:07Z
dc.date.issued2016-05-03
dc.description.abstractIncorporation into cell membranes is key for the action of photosensitizers in photomedicine treatments, with hydroperoxidation as the prominent pathway of lipid oxidation. In this paper, we use Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as cell membrane models to investigate adsorption of the photosensitizer erythrosin and its effect on photoinduced lipid oxidation. From surface pressure isotherms and polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) data, erythrosin was found to adsorb mainly via electrostatic interaction with the choline in the head groups of both DOPC and DPPC. It caused larger monolayer expansion in DOPC, with possible penetration into the hydrophobic unsaturated chains, while penetration into the DPPC saturated chains was insignificant. Easier penetration is due to the less packed DOPC monolayer, in comparison to the more compact DPPC according to the monolayer compressibility data. Most importantly, light irradiation at 530 nm made the erythrosin-containing DOPC monolayer become less unstable, with a relative surface area increase of ca. 19%, in agreement with previous findings for bioadhesive giant vesicles. The relative area increase is consistent with hydroperoxidation, supporting the erythrosin penetration into the lipid chains, which favors singlet oxygen generation close to double bonds, an important requirement for photodynamic efficiency.en
dc.description.affiliationIFSC São Carlos Institute of Physics University of São Paulo (USP)
dc.description.affiliationDCB Faculdade de Ciências e Letras UNESP Univ Estadual Paulista
dc.description.affiliationDF Faculdade de Ciências e Tecnologia UNESP Univ Estadual Paulista
dc.description.affiliationUnespDCB Faculdade de Ciências e Letras UNESP Univ Estadual Paulista
dc.description.affiliationUnespDF Faculdade de Ciências e Tecnologia UNESP Univ Estadual Paulista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/14262-7
dc.format.extent3766-3773
dc.identifierhttp://dx.doi.org/10.1021/acs.langmuir.6b00693
dc.identifier.citationLangmuir, v. 32, n. 15, p. 3766-3773, 2016.
dc.identifier.doi10.1021/acs.langmuir.6b00693
dc.identifier.issn1520-5827
dc.identifier.issn0743-7463
dc.identifier.lattes7384168674539702
dc.identifier.orcid0000-0003-4701-6408
dc.identifier.scopus2-s2.0-84966309312
dc.identifier.urihttp://hdl.handle.net/11449/178003
dc.language.isoeng
dc.relation.ispartofLangmuir
dc.relation.ispartofsjr1,479
dc.relation.ispartofsjr1,479
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleMolecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosinen
dc.typeArtigo
unesp.author.lattes7384168674539702[1]
unesp.author.lattes6118325967319836[5]
unesp.author.orcid0000-0003-4701-6408[1]
unesp.author.orcid0000-0002-5921-3161[5]

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