Anti-platelet activity of infusion of Tithonia diversifolia's leaves

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dc.contributor.authorSoares, V. C G
dc.contributor.authorBaldissera, L.
dc.contributor.authorDiz-Filho, E. B S
dc.contributor.authorAntunes, E.
dc.contributor.authorToyamaM, M. H. [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUNIP
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:56Z
dc.date.available2014-05-27T11:26:56Z
dc.date.issued2012-08-20
dc.description.abstractTithonia diversifolia, also known as Mexican arnica, has been used in traditional medicine to treat inflammatory refractory with absence of citotoxicity. The possible health risks associated with the consumption of ingestion of the infusion (tea) plant makes it is necessary to identify the potential pharmacological activity or toxicity to prove certain plants that are acclimated in Brazil. Considering the limited number of pharmacological studies regarding the Tithonia diversifolia, the aim of this study was evaluate the effects of this infusion in platelet aggregation. Venous blood was collected with informed consent from healthy volunteers who denied taking any medication in the previous 14 days. Whole blood was transferred into polypropylene tubes containing one-tenth of final volume of acid citrate dextrose (ACD-C; citric acid 3%, trisodium citrate 4%, glucose 2%; 1:9 v/v) and centrifuged at 200g for 15 min. Platelet rich plasma was added of wash buffer solution (NaCl 140mM, KCl 5mM, sodium citrate 12mM, glucose 10mM and saccharose 12mM; pH 6; 5:7 v/v) and centrifuged at 800g for 12 min at 20°C. Platelet pellet was gently resuspended in Krebs-Ringer solution and counts were performed on a Neubauer chamber. Aggregation assay was carried out with 400 μL of platelet suspension (1.2x10 8 platelets/mL) in a cuvette at 37°C with constant stirring. Platelet suspension was incubated for 3 min with aqueous extract infusion (0.6-20μg/mL) prior to addition of thrombin (100 mU/mL). Percentage of platelet aggregation was recorded with an aggregometer (Chrono-log Lumi-Aggregometer model 560-Ca, USA). Our results show an inhibition of thrombin induced platelet aggregation in the presence of 0.6-20 ug/mL Tithonia diversifolia infusion leaves. The Tithonia diversifolia infusion leaves inhibits thrombin induced washed platelet aggregation.en
dc.description.affiliationDepartment of Biochemistry Institute of Biology UNICAMP, Campinas, SP
dc.description.affiliationInstitute of Health Sciences College of Pharmacy UNIP, Jundiai, SP
dc.description.affiliationDepartment of Pharmacology Faculty of Medical Sciences UNICAMP, Campinas, SP
dc.description.affiliationExperimental Paulista Campus UNESP, Sao Vicente, SP
dc.description.affiliationUnespExperimental Paulista Campus UNESP, Sao Vicente, SP
dc.format.extent124-127
dc.identifierhttp://pharmacologyonline.silae.it/files/specialissues/2012/vol1/PhOL_2012_1_S020_Soares.pdf
dc.identifier.citationPharmacologyonline, v. 1, n. SPL. 1, p. 124-127, 2012.
dc.identifier.issn1827-8620
dc.identifier.scopus2-s2.0-84865033939
dc.identifier.urihttp://hdl.handle.net/11449/73500
dc.language.isoeng
dc.relation.ispartofPharmacologyonline
dc.relation.ispartofsjr0,163
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAqueous extract
dc.subjectPlatelet aggregation
dc.subjectTithonia diversifolia
dc.subjectacid citrate dextrose
dc.subjectantithrombocytic agent
dc.subjectcitrate sodium
dc.subjectglucose
dc.subjectplant extract
dc.subjectpolypropylene
dc.subjectpotassium chloride
dc.subjectsodium chloride
dc.subjectsucrose
dc.subjectthrombin
dc.subjectTithonia diversifolia extract
dc.subjectunclassified drug
dc.subjectanalytical equipment
dc.subjectantiplatelet activity
dc.subjectcentrifugation
dc.subjectconcentration response
dc.subjectdrug activity
dc.subjectdrug infusion
dc.subjecthuman
dc.subjecthuman cell
dc.subjectincubation time
dc.subjectinformed consent
dc.subjectmedicinal plant
dc.subjectpH
dc.subjectplant leaf
dc.subjectthrombocyte aggregation inhibition
dc.subjectthrombocyte count
dc.subjectthrombocyte rich plasma
dc.subjectvenous blood
dc.titleAnti-platelet activity of infusion of Tithonia diversifolia's leavesen
dc.typeArtigo
dcterms.licensehttp://pharmacologyonline.silae.it/subrules
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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