Functional profile of antigen specific CD4+ T Cells in the Immune response to phospholipase a1 allergen from polybia paulista Venom

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dc.contributor.authorFernandes, Lu s Gustavo Romani
dc.contributor.authorPerez-Riverol, Amilcar
dc.contributor.authorBazon, Murilo Luiz [UNESP]
dc.contributor.authorAbram, D bora Moitinho
dc.contributor.authorBrochetto-Braga, M rcia Regina [UNESP]
dc.contributor.authorZollner, Ricardode Lima
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionS o Paulo State University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:10:46Z
dc.date.available2020-12-12T02:10:46Z
dc.date.issued2020-06-01
dc.description.abstractInsect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN- and IL-6, and no significant differences to IL-4, IL-1 and TGF- 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.en
dc.description.affiliationLaboratory of Translational Immunology Medicine School FCM University of Campinas
dc.description.affiliationCenter of the Study of Social Insects Department of General and Applied Biology Institute of Biosciences of Rio Claro Institute of Biosciences of Rio Claro S o Paulo State University
dc.description.affiliationLaboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University
dc.description.affiliationVenoms and Venomous Animal Studies Center CEVAP S o Paulo State University
dc.description.affiliationUnespLaboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University
dc.identifierhttp://dx.doi.org/10.3390/toxins12060379
dc.identifier.citationToxins, v. 12, n. 6, 2020.
dc.identifier.doi10.3390/toxins12060379
dc.identifier.issn2072-6651
dc.identifier.scopus2-s2.0-85086356602
dc.identifier.urihttp://hdl.handle.net/11449/200595
dc.language.isoeng
dc.relation.ispartofToxins
dc.sourceScopus
dc.subjectCD4+ T cells
dc.subjectHymenoptera Venom Allergy
dc.subjectPolybia Paulista
dc.titleFunctional profile of antigen specific CD4+ T Cells in the Immune response to phospholipase a1 allergen from polybia paulista Venomen
dc.typeArtigo

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