Leishmanicidal activity of the crude extract, fractions and major piperidine alkaloids from the flowers of Senna spectabilis

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dc.contributor.authorAlbuquerque Melo, Gabriela Muniz de
dc.contributor.authorRodrigues Silva, Marcela Campelo
dc.contributor.authorGuimaraes, Thais Pereira
dc.contributor.authorPinheiro, Katia Mantovani
dc.contributor.authorBrito da Matta, Carolina Barbosa
dc.contributor.authorQueiroz, Aline Cavalcanti de
dc.contributor.authorPivatto, Marcos [UNESP]
dc.contributor.authorBolzani, Vanderlan da Silva [UNESP]
dc.contributor.authorAlexandre-Moreira, Magna Suzana
dc.contributor.authorViegas, Claudio
dc.contributor.institutionUniversidade Federal de Alagoas (UFAL)
dc.contributor.institutionUniversidade Federal de Alfenas (UNIFAL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:48Z
dc.date.available2014-12-03T13:10:48Z
dc.date.issued2014-02-15
dc.description.abstractSenna spectabilis (sin. Cassia excelsa, C. spectabilis) is an endemic tree of South America and Africa, very common in Brazil, where it is known as canafistula-de-besouro and cassia-do-nordeste. In folk medicine, this plant is indicated for the treatment of constipation, insomnia, anxiety, epilepsy, malaria, dysentery and headache. Phytopharmacological studies have also confirmed anticonvulsive, sedative, anti-malarial, antimicrobial and cytotoxic properties of many parts of S. spectabilis. In this communication, we present a comparative study of the leishmanicidal activity of the crude ethanolic extract, its fractions and also the two major alkaloidal metabolites (-)-cassine/(-)-spectaline, trying to establish a relationship between the presence of piperidine alkaloidal constituents and leishmanicidal activity. The growth inhibitory effect of promastigote forms of Leishmania major was determined for the crude extract, fractions of the flowers of S. spectabilis and a mixture of (-)-cassine/(-)-spectaline in comparison to pentamidine used as standard drug. The cytotoxic effects were assessed on macrophage strain J774 by lactate dehydrogenase assay. Fractions dichloromethane (FL-DCM) and n-butanol (FL-Bu) and a mixture of (-)-cassine/(-)-spectaline (similar to 7:3) exhibited significant activity against the parasite Leishmania major (IC50 values of 0.6 +/- 0.1 mu g/ml, 1.6 +/- 0.9 mu g/ml and 24.9 +/- 1.4 mu g/ml, respectively), without toxic effects on murine macrophages. Due to the promising results elicited, further studies in vivo need to be performed to confirm the therapeutic potential of Senna spectabilis. (C) 2013 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniv Fed Alagoas, Ctr Ciencias Biol, Lab Farmacol & Imunidade, BR-57020720 Maceio, AL, Brazil
dc.description.affiliationUniv Fed Alfenas, Inst Quim, LFQM, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Nucl Bioensaios Biossintese & Ecofisiol Prod Nat, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Nucl Bioensaios Biossintese & Ecofisiol Prod Nat, BR-14801970 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipFAPEAL
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFINEP
dc.format.extent277-281
dc.identifierhttp://dx.doi.org/10.1016/j.phymed.2013.09.024
dc.identifier.citationPhytomedicine. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 21, n. 3, p. 277-281, 2014.
dc.identifier.doi10.1016/j.phymed.2013.09.024
dc.identifier.issn0944-7113
dc.identifier.urihttp://hdl.handle.net/11449/112524
dc.identifier.wosWOS:000334012400012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPhytomedicine
dc.relation.ispartofjcr3.610
dc.relation.ispartofsjr1,087
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAntiprotozoal activityen
dc.subjectLeishmanicidal activityen
dc.subjectSenna spectabilisen
dc.subjectCassia spectabilisen
dc.subjectPiperidine alkaloidsen
dc.titleLeishmanicidal activity of the crude extract, fractions and major piperidine alkaloids from the flowers of Senna spectabilisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-7799-992X[10]
unesp.author.orcid0000-0001-7019-5825[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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