Decreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis

Página do item simplificado
dc.contributor.authorMarcos, Caroline Maria [UNESP]
dc.contributor.authorda Silva, Julhiany de Fátima [UNESP]
dc.contributor.authorde Oliveira, Haroldo Cesar [UNESP]
dc.contributor.authorAssato, Patrícia Akemi [UNESP]
dc.contributor.authorSingulani, Junya de Lacorte [UNESP]
dc.contributor.authorLopez, Angela Maria
dc.contributor.authorTamayo, Diana Patricia
dc.contributor.authorHernandez-Ruiz, Orville
dc.contributor.authorMcEwen, Juan G.
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorFusco-Almeida, Ana Marisa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCorporación para Investigaciones Biológicas (CIB) - Medellín
dc.contributor.institutionUniversidad de Antioquia
dc.date.accessioned2018-12-11T17:27:36Z
dc.date.available2018-12-11T17:27:36Z
dc.date.issued2016-02-17
dc.description.abstractThe interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 protein. The 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. Here, we investigated the relevance of the 14-3-3 protein to the virulence of P. brasiliensis. Using antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated a 14-3-3-silenced strain (expression reduced by ˜55%). This strain allowed us to investigate the interaction between 14-3-3 and the host and to correlate the functions of P. brasiliensis 14-3-3 with cellular features, such as morphological characteristics and virulence, that are important for pathogenesis.en
dc.description.affiliationUNESP - Univ Estadual Paulista Departamento de Análises Clínicas; Laboratório de Micologia Clínica
dc.description.affiliationUnidad de Biología Celular y Molecular Corporación para Investigaciones Biológicas (CIB) - Medellín
dc.description.affiliationEscuela de Microbiología Universidad de Antioquia
dc.description.affiliationUniversidad de Antioquia
dc.description.affiliationUnespUNESP - Univ Estadual Paulista Departamento de Análises Clínicas; Laboratório de Micologia Clínica
dc.format.extent72-84
dc.identifierhttp://dx.doi.org/10.1080/21505594.2015.1122166
dc.identifier.citationVirulence, v. 7, n. 2, p. 72-84, 2016.
dc.identifier.doi10.1080/21505594.2015.1122166
dc.identifier.file2-s2.0-84961637959.pdf
dc.identifier.file2-s2.0-84961637959.pdf
dc.identifier.issn2150-5608
dc.identifier.issn2150-5594
dc.identifier.scopus2-s2.0-84961637959
dc.identifier.urihttp://hdl.handle.net/11449/177894
dc.language.isoeng
dc.relation.ispartofVirulence
dc.relation.ispartofsjr1,635
dc.relation.ispartofsjr1,635
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject14-3-3 protein
dc.subjectgene knockdown
dc.subjectmorphological alterations
dc.subjectParacoccidioides brasiliensis
dc.subjectvirulence
dc.titleDecreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesisen
dc.typeArtigo
unesp.author.lattes3716273524139678[11]
unesp.author.orcid0000-0002-2115-8988[11]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-84961637959.pdf
Tamanho:
1.06 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Análises Clínicas - FCFAR