Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)

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dc.contributor.authorSannomiya, M.
dc.contributor.authorRodrigues, C. M.
dc.contributor.authorCoelho, R. G.
dc.contributor.authorSantos, Lourdes Campaner dos [UNESP]
dc.contributor.authorHiruma-Lima, C. A.
dc.contributor.authorBrito, ARS
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:49:47Z
dc.date.available2014-05-20T13:49:47Z
dc.date.issued2004-04-30
dc.description.abstractThe methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by H-1 NMR, C-13 NMR and UV analyses. (C) 2004 Published by Elsevier B.V.en
dc.description.affiliationUNESP, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fisiol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNICAMP, Inst Biol, Dept Fisiol & Biofis, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fisiol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent47-51
dc.identifierhttp://dx.doi.org/10.1016/j.chroma.2004.02.050
dc.identifier.citationJournal of Chromatography A. Amsterdam: Elsevier B.V., v. 1035, n. 1, p. 47-51, 2004.
dc.identifier.doi10.1016/j.chroma.2004.02.050
dc.identifier.issn0021-9673
dc.identifier.lattes3538253640602977
dc.identifier.lattes3814504901386844
dc.identifier.orcid0000-0002-8645-3777
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/17758
dc.identifier.wosWOS:000220790800007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography A
dc.relation.ispartofjcr3.716
dc.relation.ispartofsjr1,378
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcounter-current chromatographypt
dc.subjectByrsonima crassapt
dc.subjectplant materialspt
dc.subjectflavonoidspt
dc.subjectamentoflavonept
dc.titleApplication of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3538253640602977
unesp.author.lattes3814504901386844[5]
unesp.author.orcid0000-0002-8645-3777[5]
unesp.author.orcid0000-0003-3032-2556[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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Artigos - Química Orgânica - IQ
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