Upregulation of mRNA myocardium calcium handling in rats submitted to exercise and food restriction

Página do item simplificado
dc.contributor.authorSugizaki, Mário Mateus [UNESP]
dc.contributor.authorLeopoldo, Ana Paula Lima [UNESP]
dc.contributor.authorConde, Sandro José [UNESP]
dc.contributor.authorCampos, Dijon Salome [UNESP]
dc.contributor.authorDamato, Ricardo [UNESP]
dc.contributor.authorLeopoldo, André Soares [UNESP]
dc.contributor.authordo Nascimento, André Ferreira [UNESP]
dc.contributor.authorJúnior, Silvio de Assis Oliveira [UNESP]
dc.contributor.authorCicogna, Antonio Carlos [UNESP]
dc.contributor.institutionInstituto de Ciências da Saúde - Universidade Federal de Mato Grosso - UFMT
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:48:45Z
dc.date.available2022-04-29T08:48:45Z
dc.date.issued2011-07-01
dc.description.abstractBackground: Chronic exercise and food restriction (FR) have directionally opposite changes in transcription of molecular structures of calcium handling and thyroid hormone (TH) status. Objective: Evaluate the association of chronic exercise and FR on serum thyroid hormones and gene transcription of molecular structures of intracellular calcium transients and thyroid receptors in myocardium of rats. Methods: Male Wistar Kyoto rats, divided into two groups: control (C, n = 7), FR (R50', n = 7), chronic exercise (EX, n = 7) and chronic exercise + FR (EX50', n = 7). FR was of 50% and exercise was swimming (1 hour/day, 5 days/ week, during 12 weeks). Serum concentrations of T3', T4 and TSH were determined. The mRNA gene expression of the sarcoplasmatic reticulum calcium pump (SERCA2a), phospholamban (PLB), Na+/Ca+2 exchanger (NCX), calcium channel L-type (L-channel), ryanodine (RYR), calsequestrin (CQS) and HT receptor (TRα1 and TRβ1) of the myocardium was performed by PCR real-time. Results: FR reduced serum levels of T4 and TSH and TRα1 mRNA and increased the expression of PLB, NCX and L-channel. Exercise increased the TRβ1 receptor, L-channel and NCX. The association of exercise and FR reduced plasma T4 and TSH, TRβ1 mRNA increase, SERCA2a, NCX and PLB, and there was a significant correlation of TRβ1 with CQS and NXC. Conclusion: Chronic exercise and food restriction increased the mRNA of transient Ca2+ proteins; however, TH - receptor axis cannot participate in the transcription of mRNA of myocardial calcium transient proteins.en
dc.description.affiliationInstituto de Ciências da Saúde - Universidade Federal de Mato Grosso - UFMT Campus de Sinop, Botucatu
dc.description.affiliationCuiabá, MT Faculdade de Medicina - Universidade Estadual Paulista - UNESP Campus de Botucatu, Botucatu
dc.description.affiliationUnespCuiabá, MT Faculdade de Medicina - Universidade Estadual Paulista - UNESP Campus de Botucatu, Botucatu
dc.identifier.citationArquivos Brasileiros de Cardiologia, v. 97, n. 1, 2011.
dc.identifier.issn0066-782X
dc.identifier.issn1678-4170
dc.identifier.scopus2-s2.0-80052758480
dc.identifier.urihttp://hdl.handle.net/11449/232028
dc.language.isoeng
dc.relation.ispartofArquivos Brasileiros de Cardiologia
dc.sourceScopus
dc.subjectCalcium
dc.subjectCaloric restriction
dc.subjectCarrier proteins
dc.subjectExercise
dc.subjectMyocardium
dc.subjectThyroid hormones
dc.titleUpregulation of mRNA myocardium calcium handling in rats submitted to exercise and food restrictionen
dc.typeArtigo

Arquivos

Coleções

Artigos - Clínica Médica - FMB