Ethanolic extract of Casearia sylvestris and its clerodane diterpen (caseargrewiin F) protect against DNA damage at low concentrations and cause DNA damage at high concentrations in mice's blood cells

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dc.contributor.authorde Oliveira, Aline M. [UNESP]
dc.contributor.authorSantos, André Gonzaga dos [UNESP]
dc.contributor.authordos Santos, Raquel A.
dc.contributor.authorCsipak, Angelica R. [UNESP]
dc.contributor.authorOlivato, Camila [UNESP]
dc.contributor.authorda Silva, Isabel C. [UNESP]
dc.contributor.authorde Freitas, Maura B. [UNESP]
dc.contributor.authorBassi, Carmen L.
dc.contributor.authorCavalheiro, Alberto José [UNESP]
dc.contributor.authorBolzani, Vanderlan da Silva [UNESP]
dc.contributor.authorSilva, Dulce H. S. [UNESP]
dc.contributor.authorSakamoto-Hojo, Elza T.
dc.contributor.authorTakahashi, Catarina S.
dc.contributor.authorSoares, Christiane P. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T14:20:04Z
dc.date.available2014-05-20T14:20:04Z
dc.date.issued2009-11-01
dc.description.abstractCasearia sylvestris is used in Brazil as a popular medicine to treat ulcer, inflammation and tumour. Caseargrewiin F is a clerodane diterpene isolated from the ethanolic leaf extract of C.sylvestris. The aim of the study was to assess the capacity of the ethanolic extract of C.sylvestris leaves and caseargrewiin F to protect DNA and verify if both the compounds cause some DNA damage, using the micronucleus (MN) test and comet assay in mice. Balb-C mice were treated with the extract [3.13, 6.25, 12.5, 25, 50 and 75 mg/kg body weight (b.w.)] and caseargrewiin F (0.16, 0.32, 0.63, 1.3, 2.5 and 3.8 mg/kg b.w.) for 14 days. on day 15, DNA damage was induced by intra-peritoneal (i.p.) injection of cyclophosphamide (CP) (i.p.) at 50 mg/kg b.w. after the MN test and comet assay were performed. A protective effect of ethanolic extract was observed in MN test (6.25 and 12.5 mg/kg b.w.) and the comet assay (3.13 and 6.25, 12.5 and 25 mg/kg b.w.). Caseargrewiin F showed protective effect at 0.63, 1.3 and 2.5 mg/kg b.w. only in comet assay. We also tested the ability of compounds of C.sylvestris to induce MN and to increase the comet assay tail moment. The experimental design was similar to the DNA protection assay except that in test groups we omitted the CP challenge. We observed increased damage at 50 and 75 mg/kg b.w. of ethanolic extract of C.sylvestris and caseargrewiin F at 3.18 mg/kg b.w. in both the MN test and comet assay. We conclude that ethanolic extract of C. sylvestris and caseargrewiin F can protect cells against DNA damage induced by CP at low concentrations, but at high concentrations these compounds also induce DNA damage.en
dc.description.affiliationSão Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Sch Pharmaceut Sci, Dept Principios Ativos Nat & Toxicol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Biol, Fac Philosophy Sci & Letters Ribeirao Preto, BR-05508 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Inst Chem, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmaceut Sci, Dept Principios Ativos Nat & Toxicol, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Inst Chem, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBioprospecta
dc.description.sponsorshipConselho Nacional de Desenvolvimento a Pesquisa
dc.description.sponsorshipIdFAPESP: 05/58472-9
dc.description.sponsorshipIdFAPESP: 03/02176-7
dc.description.sponsorshipIdBioprospecta: 2004/07932-7
dc.description.sponsorshipIdCNPq: 305615/2006-8
dc.format.extent501-506
dc.identifierhttp://dx.doi.org/10.1093/mutage/gep034
dc.identifier.citationMutagenesis. Oxford: Oxford Univ Press, v. 24, n. 6, p. 501-506, 2009.
dc.identifier.doi10.1093/mutage/gep034
dc.identifier.issn0267-8357
dc.identifier.lattes4702004904231248
dc.identifier.lattes1768025290373669
dc.identifier.orcid0000-0002-1516-7765
dc.identifier.orcid0000-0003-1740-7360
dc.identifier.urihttp://hdl.handle.net/11449/26023
dc.identifier.wosWOS:000271575700006
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofMutagenesis
dc.relation.ispartofjcr2.840
dc.relation.ispartofsjr0,916
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleEthanolic extract of Casearia sylvestris and its clerodane diterpen (caseargrewiin F) protect against DNA damage at low concentrations and cause DNA damage at high concentrations in mice's blood cellsen
dc.typeArtigo
dcterms.licensehttp://maneypublishing.com/index.php/resources/authors/copyright_and_permissions_information_for_authors/
dcterms.rightsHolderOxford Univ Press
unesp.author.lattes4702004904231248[11]
unesp.author.lattes2518006820764120[9]
unesp.author.lattes4000625974516852[2]
unesp.author.lattes1768025290373669[14]
unesp.author.orcid0000-0002-4920-2506[2]
unesp.author.orcid0000-0002-1516-7765[11]
unesp.author.orcid0000-0001-8214-9957[9]
unesp.author.orcid0000-0003-1740-7360[14]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Análises Clínicas - FCFAR
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