Effects of antiproteolytic agents on corneal epithelial viability and matrix metalloproteinase-2 and metalloproteinase-9 activity in alkali-burned corneas of rats

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dc.contributor.authorTrujillo Piso, Dunia Y. [UNESP]
dc.contributor.authorRibeiro, Alexandre P.
dc.contributor.authorSilva, Miguel L. [UNESP]
dc.contributor.authorGuimaraes, Patricia J. [UNESP]
dc.contributor.authorMorales, Adriana [UNESP]
dc.contributor.authorMartins, Bianca C. [UNESP]
dc.contributor.authorPadua, Ivan M. [UNESP]
dc.contributor.authorRenzo, Roberta [UNESP]
dc.contributor.authorAndrade, Alexandre L. [UNESP]
dc.contributor.authorUscategui, Ricardo R. [UNESP]
dc.contributor.authorLaus, Jose L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Fed Mato Grosso
dc.date.accessioned2014-12-03T13:10:45Z
dc.date.available2014-12-03T13:10:45Z
dc.date.issued2014-01-01
dc.description.abstractObjective To evaluate the effects of agents on corneal re-epithelization and metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) activities in corneas of rats submitted to ulceration.Animals Studied Ninety eight healthy rats.Procedures Corneal ulcers were created using 1N NaOH in their left eye. Eyes were treated every 6 h with 1% ethylenediaminetetraacetic acid (EDTA), 3% chondroitin sulfate (CS), 10% N-acetylcysteine NAc and saline (S) at 6-h intervals. Corneas were stained with fluorescein and photographed at the same time points. Following 20 h and 40-42 h of corneal injury, corneas were processed for scanning electron microscopy (SEM) to quantify microvilli density, and MMPs activities were analyzed using zymography.Results The percentage of wound area and the time in hours for corneal re-epithelization did not differ significantly among treatment groups (P > 0.05). In first and the second moments, latent MMP-2 was significantly elevated in the eyes treated with NAC and CS (P < 0.001). Active MMP-2 did not change significantly among treatment groups in the first moment (P > 0.05); significantly higher activity was observed in the second moment in the eyes treated with CS (P < 0.001). In the second moment, latent MMP-9 decreased significantly in eyes treated with EDTA and S (P < 0.01). Microvilli corneal density did not change significantly between healthy subjects and treatment groups (P > 0.05).Conclusion Any of the studied substances did not accelerate corneal re-epithelization and did not add protection to the corneal microvilli. Significant higher levels of active form of MMP-2 in 3% chondroitin sulfate-treated group may indicate that the agent acts as substrate for such enzyme. At the end of the experiment, 1% EDTA was the most efficient agent to inhibit significantly the latent form of MMP-9. However, any of the substances add benefit over saline on reducing the proteolytic activity in the cornea of rats after alkali injury.en
dc.description.affiliationSao Paulo State Univ, Coll Agr Sci & Vet Med, Dept Vet Clin & Surg, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Fed Mato Grosso, Coll Agron Vet Med & Anim Sci, Dept Vet Clin, Cuiaba, MT, Brazil
dc.description.affiliationSao Paulo State Univ, Coll Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Coll Agr Sci & Vet Med, Dept Vet Clin & Surg, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Coll Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent23-31
dc.identifierhttp://dx.doi.org/10.1111/vop.12032
dc.identifier.citationVeterinary Ophthalmology. Hoboken: Wiley-blackwell, v. 17, n. 1, p. 23-31, 2014.
dc.identifier.doi10.1111/vop.12032
dc.identifier.issn1463-5216
dc.identifier.lattes6404176495306171
dc.identifier.lattes4786199173732793
dc.identifier.orcid0000-0001-8967-0753
dc.identifier.urihttp://hdl.handle.net/11449/112486
dc.identifier.wosWOS:000330952200005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofVeterinary Ophthalmology
dc.relation.ispartofjcr1.083
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCorneaen
dc.subjectmetalloproteinasesen
dc.subjectRaten
dc.subjectulcerative keratitisen
dc.titleEffects of antiproteolytic agents on corneal epithelial viability and matrix metalloproteinase-2 and metalloproteinase-9 activity in alkali-burned corneas of ratsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes6404176495306171[9]
unesp.author.lattes4786199173732793[5]
unesp.author.orcid0000-0002-9760-8631[10]
unesp.author.orcid0000-0002-8922-2886[2]
unesp.author.orcid0000-0001-8967-0753[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Clínica e Cirurgia Veterinária - FCAV
Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA