Intrauterine blood plasma platelet-therapy mitigates persistent breeding-induced endometritis, reduces uterine infections, and improves embryo recovery in Mares

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dc.contributor.authorSegabinazzi, Lorenzo G. T. M. [UNESP]
dc.contributor.authorCanisso, Igor F.
dc.contributor.authorPodico, Giorgia
dc.contributor.authorCunha, Lais L.
dc.contributor.authorNovello, Guilherme [UNESP]
dc.contributor.authorRosser, Michael F.
dc.contributor.authorLoux, Shavahn C.
dc.contributor.authorLima, Fabio S.
dc.contributor.authorAlvarenga, Marco A. [UNESP]
dc.contributor.institutionUniversity of Illinois Urbana Champaign
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Kentucky
dc.contributor.institutionRoss University School of Veterinary Medicine
dc.contributor.institutionUniversity of California
dc.date.accessioned2021-06-25T10:59:23Z
dc.date.available2021-06-25T10:59:23Z
dc.date.issued2021-05-01
dc.description.abstractMicroorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or-poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre-(−48 and −24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Ilβ, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey’s post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property.en
dc.description.affiliationDepartment of Veterinary Clinical Medicine College of Veterinary Medicine University of Illinois Urbana Champaign, 1008 W Hazelwood Drive
dc.description.affiliationDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.affiliationVeterinary Diagnostic Laboratory College of Veterinary Medicine University of Illinois Urbana Champaign
dc.description.affiliationMaxwell H. Gluck Equine Research Center University of Kentucky
dc.description.affiliationRoss University School of Veterinary Medicine, PO Box 334
dc.description.affiliationDepartment of Population Health and Reproduction School of Veterinary Medicine University of California
dc.description.affiliationUnespDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipU.S. Department of Agriculture
dc.description.sponsorshipIdFAPESP: 2018/02856-3
dc.description.sponsorshipIdU.S. Department of Agriculture: ILLU-888-912
dc.identifierhttp://dx.doi.org/10.3390/antibiotics10050490
dc.identifier.citationAntibiotics, v. 10, n. 5, 2021.
dc.identifier.doi10.3390/antibiotics10050490
dc.identifier.issn2079-6382
dc.identifier.scopus2-s2.0-85105288541
dc.identifier.urihttp://hdl.handle.net/11449/207693
dc.language.isoeng
dc.relation.ispartofAntibiotics
dc.sourceScopus
dc.subjectEndometrium
dc.subjectEquine
dc.subjectImmunomodulation
dc.subjectPRP
dc.subjectUterine inflammation
dc.titleIntrauterine blood plasma platelet-therapy mitigates persistent breeding-induced endometritis, reduces uterine infections, and improves embryo recovery in Maresen
dc.typeArtigo

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