Absence of carcinogenic and anticarcinogenic effects of annatto in the rat liver medium-term assay

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dc.contributor.authorAgner, A. R.
dc.contributor.authorBarbisan, Luis Fernando [UNESP]
dc.contributor.authorScolastici, C.
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:00Z
dc.date.available2014-05-20T13:37:00Z
dc.date.issued2004-10-01
dc.description.abstractAnnatto (Bixa orellana L.) is a natural food colorant extensively used in many processed foods, especially dairy products. The lower cost of production and the low toxicity, make annatto a very attractive and convenient pigment in substitution to the many synthetic colorants. In the present study we investigate the carcinogenic and anticarcinogenic effects of dietary annatto in Wistar rat liver using the preneoplastic glutathione S-transferase (GST-P) foci and DNA damage biomarkers. Annatto, containing 5% bixin, was administered in the diet at concentrations of 20, 200, and 1000 ppm (0.07; 0.80 and 4.23 bixin/kg body wt/day, respectively), continuously during 2 weeks before, or 8 weeks after DEN treatment (200 mg/kg body wt, i.p.), to evaluate its effect on the liver-carcinogenesis medium-term bioassay. The comet assay was used to investigate the modifying potential of annatto on DEN (20 mg/kg body wt)-induced DNA damage. The results showed that annatto was neither genotoxic nor carcinogenic at the highest concentration tested (1000 ppm). No protective effects were also observed in both GST-P foci development and comet assays. In conclusion, in such experimental conditions, annatto shows no hepatocarcinogenic effect or modifying potential against DEN-induced DNA damage and preneoplastic foci in the rat liver. (C) 2004 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Patol, TOXICAN,Nucleo Avaliacao Tox Genet & Canc Igena, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Fac Med, Dept Patol, TOXICAN,Nucleo Avaliacao Tox Genet & Canc Igena, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent1687-1693
dc.identifierhttp://dx.doi.org/10.1016/j.fct.2004.06.005
dc.identifier.citationFood and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 42, n. 10, p. 1687-1693, 2004.
dc.identifier.doi10.1016/j.fct.2004.06.005
dc.identifier.issn0278-6915
dc.identifier.lattes3278528112652257
dc.identifier.lattes5051118752980903
dc.identifier.orcid0000-0002-2180-1814
dc.identifier.urihttp://hdl.handle.net/11449/12768
dc.identifier.wosWOS:000223643900015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFood and Chemical Toxicology
dc.relation.ispartofjcr3.977
dc.relation.ispartofsjr1,144
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectannattopt
dc.subjectcarcinogenesispt
dc.subjectchemopreventionpt
dc.subjectdiethylnitrosaminept
dc.subjectgenotoxicitypt
dc.subjectGST-P positive focipt
dc.subjectcomet assaypt
dc.titleAbsence of carcinogenic and anticarcinogenic effects of annatto in the rat liver medium-term assayen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3278528112652257[2]
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0001-9323-3134[4]
unesp.author.orcid0000-0002-2180-1814[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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