High prevalence of aggregative adherent Escherichia coli strains in the mucosa-associated microbiota of patients with inflammatory bowel diseases

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dc.contributor.authorThomazini, Cristiane M. [UNESP]
dc.contributor.authorSamegima, Danielle A. G. [UNESP]
dc.contributor.authorRodrigues, Maria Aparecida Marchesan [UNESP]
dc.contributor.authorVictoria, Carlos R. [UNESP]
dc.contributor.authorRodrigues, Josias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:37:18Z
dc.date.available2014-05-20T13:37:18Z
dc.date.issued2011-08-01
dc.description.abstractThe intestinal population of Escherichia coil is increased in patients with inflammatory bowel disease (IBD), but the reason for this elevation, the particular features of these bacteria and their potential role in the pathogenesis of the disease are not known. The present study was undertaken to investigate the adherence abilities and some virulence properties of a collection of 131 E. coil isolates cultured from rectal biopsies of 23 subjects diagnosed with ulcerative colitis (UC), 8 with Crohn's disease (CD) and 23 control patients from southern Brazil. The adherence abilities of the bacteria were investigated in vitro, using HEp-2 epithelial cells in assays of 3 and 6 h of bacteria-cell contact. The isolates were screened by PCR with primers for the following virulence genetic markers: plasmid of aggregative adhesion (pAA) and the aggregative adherence fimbriae R (aggR), E. coli attaching and effacing (eae), invasion-associated locus (ial), invasion plasmid antigen H (ipaH) and Shiga citotoxin-encoding (stx) genes. HEp-2 cells aggregative adherent E. coil strains, as detected in the 3 h adherence assay, were found in 14/23(60.9%) patients with UC, 7/8(87.5%) with CD and in 7/23 (30.4%) controls (p = 0.011). Virulence genetic markers were detected in strains of 9 patients with UC (39.1%), but in none of CD or control group. Two of these UC patients had strains harboring both pAA and aggR, one had strains positive for aggR, four had strains positive for eae and two had strains positive for stx. These results suggest that the augmented population of E. coil on the rectal mucosa of IBD patients, particularly of those diagnosed with UC, is mostly comprised of aggregative adherent strains, some of which possessing classical virulence markers of E. coil. (C) 2011 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUNESP, Dept Microbiol & Immunol, IBB Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, ICB Inst Biomed Sci, Postgrad Program Microbiol, BR-09500900 São Paulo, Brazil
dc.description.affiliationUNESP, FMB Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNESP, FMB Fac Med, Dept Internal Med, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Microbiol & Immunol, IBB Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, FMB Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, FMB Fac Med, Dept Internal Med, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/10975-0
dc.format.extent475-479
dc.identifierhttp://dx.doi.org/10.1016/j.ijmm.2011.04.015
dc.identifier.citationInternational Journal of Medical Microbiology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 301, n. 6, p. 475-479, 2011.
dc.identifier.doi10.1016/j.ijmm.2011.04.015
dc.identifier.issn1438-4221
dc.identifier.lattes4211432128816409
dc.identifier.urihttp://hdl.handle.net/11449/12893
dc.identifier.wosWOS:000294584300003
dc.language.isoeng
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofInternational Journal of Medical Microbiology
dc.relation.ispartofjcr3.298
dc.relation.ispartofsjr1,717
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEscherichiaen
dc.subjectEnteroaggregativeen
dc.subjectMicrobiotaen
dc.subjectUlcerativeen
dc.subjectVirulenceen
dc.titleHigh prevalence of aggregative adherent Escherichia coli strains in the mucosa-associated microbiota of patients with inflammatory bowel diseasesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier Gmbh, Urban & Fischer Verlag
unesp.author.lattes4211432128816409
unesp.author.orcid0000-0001-8480-672X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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