A Multivariate Calibration Procedure for UV/VIS Spectrometric Monitoring of BHK-21 Cell Metabolism and Growth

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dc.contributor.authorLeme, Jaci
dc.contributor.authorFernández Núñez, Eutimio Gustavo [UNESP]
dc.contributor.authorParizotto, Leticia de Almeida
dc.contributor.authorChagas, Wagner Antonio
dc.contributor.authorSantos, Erica Salla dos
dc.contributor.authorPrestia Caricati, Aline Tojeira
dc.contributor.authorRezende, Alexandre Goncalves de
dc.contributor.authorVale da Costa, Bruno Labate
dc.contributor.authorVentini Monteiro, Daniela Cristina
dc.contributor.authorLopes Boldorini, Vera Lucia
dc.contributor.authorCalil Jorge, Soraia Attie
dc.contributor.authorAstray, Renato Mancini
dc.contributor.authorPereira, Carlos Augusto
dc.contributor.authorCaricati, Celso Pereira
dc.contributor.authorTonso, Aldo
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Pesquisas Tecnol Estado Sao Paulo SA
dc.date.accessioned2014-12-03T13:11:37Z
dc.date.available2014-12-03T13:11:37Z
dc.date.issued2014-01-01
dc.description.abstractMonitoring mammalian cell culture with UV-vis spectroscopy has not been widely explored. The aim of this work was to calibrate Partial Least Squares (PLS) models from off-line UV-vis spectral data in order to predict some nutrients and metabolites, as well as viable cell concentrations for mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Spectra of samples taken from batches performed at different dissolved oxygen concentrations (10, 30, 50, and 70% air saturation), in two bioreactor configurations and with two strategies to control pH were used to calibrate and validate PLS models. Glutamine, glutamate, glucose, and lactate concentrations were suitably predicted by means of this strategy. Especially for glutamine and glucose concentrations, the prediction error averages were lower than 0.50 +/- 0.10 mM and 2.21 +/- 0.16 mM, respectively. These values are comparable with those previously reported using near infrared and Raman spectroscopy in conjunction with PLS. However, viable cell concentration models need to be improved. The present work allows for UV-vis at-line sensor development, decrease cost related to nutrients and metabolite quantifications and establishment of fed-batch feeding schemes. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:241-248, 2014en
dc.description.affiliationInst Butantan, Lab Especial Pesquisa & Desenvolvimento Imunol V, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Escola Politecn, Dept Engn Quim, Lab Celulas Anim, BR-05508900 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Ciencias Biol, BR-19806900 Assis, SP, Brazil
dc.description.affiliationInst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo, Brazil
dc.description.affiliationInst Pesquisas Tecnol Estado Sao Paulo SA, Nucleo Bionanomanufatura, Lab Biotecnol Ind, BR-05508901 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Ciencias Biol, BR-19806900 Assis, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundacao para o Desenvolvimento Tecnologico da Engenharia (FDTE)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 10/52521-6
dc.description.sponsorshipIdCNPq: CNPq: 483009/2010-5
dc.format.extent241-248
dc.identifierhttp://dx.doi.org/10.1002/btpr.1847
dc.identifier.citationBiotechnology Progress. Hoboken: Wiley-blackwell, v. 30, n. 1, p. 241-248, 2014.
dc.identifier.doi10.1002/btpr.1847
dc.identifier.issn8756-7938
dc.identifier.urihttp://hdl.handle.net/11449/113334
dc.identifier.wosWOS:000331385800026
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBiotechnology Progress
dc.relation.ispartofjcr1.947
dc.relation.ispartofsjr0,676
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectUV-vis spectroscopyen
dc.subjectpartial least squaresen
dc.subjectBHK-21en
dc.subjectcell culture monitoringen
dc.titleA Multivariate Calibration Procedure for UV/VIS Spectrometric Monitoring of BHK-21 Cell Metabolism and Growthen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0002-7055-2762[8]
unesp.author.orcid0000-0002-2800-392X[2]
unesp.author.orcid0000-0002-6983-5298[12]
unesp.author.orcid0000-0001-8498-7348[15]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt

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