Replication of classical infectious bursal disease virus in the chicken embryo related cell line

Página do item simplificado
dc.contributor.authorCardoso, T. C.
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorPilz, D.
dc.contributor.authorTeixeira, MCB
dc.contributor.authorArns, C. W.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-02-26T17:10:16Z
dc.date.accessioned2014-05-20T13:42:20Z
dc.date.available2014-02-26T17:10:16Z
dc.date.available2014-05-20T13:42:20Z
dc.date.issued2000-06-01
dc.description.abstractInfectious bursal disease (IBD) is an acute, highly contagious viral disease. The diagnosis of IBD depends on time-consuming and costly procedures, like virus isolation on chick embryos and histopathological examination, A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), immunoperoxidase and reverse transcription polymerase chain reaction (RT-PCR) were applied in this study to detect classical IBD virus (IBDV) after three blind passages of the Lukert strain on chicken embryo related (CER) cell monolayer after different periods of infection: 6, 12, 24 and 48 h, Cytophatic effects were most evident 12 h post-infection (p.i.) but were observed at 6 h p.i. The maximum discrimination between IBDV-infected and uninfected cell suspensions obtained by the use of DAS-ELISA for virus detection corresponded to 0.597+/-0.02 and 0.010+/-0.01 after 12h p.i., respectively. The RT-PCR was performed using the set of primers A3.1 and A3.2 to amplify the VP2 region of the IBDV genome, This molecular technique demonstrated that from 6 h p.i., it was possible to detect the viral RNA. The results show that the CER cell line can be used for classical IBDV propagation, confirmed by the DAS-ELISA, immunoperoxidase and RT-PCR assay.en
dc.description.affiliationUniv Estadual Paulista, Fac Odontol, Curso Med Vet, Dept Apoio Prod & Saúde Anim, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Microbiol & Immunol, Lab Virol Anim, BR-13093970 Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol, Curso Med Vet, Dept Apoio Prod & Saúde Anim, BR-16050680 Aracatuba, SP, Brazil
dc.format.extent213-217
dc.identifierhttp://dx.doi.org/10.1080/03079450050045468
dc.identifier.citationAvian Pathology. Basingstoke: Carfax Publishing, v. 29, n. 3, p. 213-217, 2000.
dc.identifier.doi10.1080/03079450050045468
dc.identifier.issn0307-9457
dc.identifier.lattes7991082362671212
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.urihttp://hdl.handle.net/11449/14725
dc.identifier.wosWOS:000088296200004
dc.language.isoeng
dc.publisherCarfax Publishing
dc.relation.ispartofAvian Pathology
dc.relation.ispartofjcr2.054
dc.relation.ispartofsjr0,871
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleReplication of classical infectious bursal disease virus in the chicken embryo related cell lineen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderCarfax Publishing
unesp.author.lattes7991082362671212[2]
unesp.author.orcid0000-0002-7308-460X[5]
unesp.author.orcid0000-0001-5693-6148[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Apoio, Produção e Saúde Animal - FMVA