Avaliação das técnicas de RT-PCR e heminested RT-PCR em cérebros de cães com sinais neurológicos compatíveis com cinomose

Página do item simplificado
dc.contributor.authorCortez, Adriana
dc.contributor.authorSouza, Vanessa [UNESP]
dc.contributor.authorFernandes, Telma [UNESP]
dc.contributor.authorMegid, Jane [UNESP]
dc.contributor.institutionMedicina Veterinária
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:02:33Z
dc.date.available2022-04-28T19:02:33Z
dc.date.issued2015-01-01
dc.description.abstractThe diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected from 68 dogs and tested by direct immunofluorescence test (RFID) and, independent of the results, they were stored at -20°C for at least three years. They were submitted to the RT-PCR and hnRT-PCR techniques aiming to determine the gene responsible for the viral nucleoprotein decoding. Fifty-nine samples were positive for RIFD, 40 for RT-PCR (Kappa = 0.358) and 54 for hnRT-PCR (Kappa = 0.740). All nine RIFD negative samples were also negative for RT-PCR and hnRT-PCR. In spite of the storage duration and proper sample conditions, the estimated accordance between hnRT-PCR and RIFD demonstrated that hnRT-PCR technique can be applied in retrospective studies.en
dc.description.affiliationUniversidade de Santo Amaro Medicina Veterinária
dc.description.affiliationUniversidade Estadual Paulista
dc.description.affiliationUnespUniversidade Estadual Paulista
dc.format.extent363-365
dc.identifierhttp://dx.doi.org/10.11606/issn.l6784456.v52i4p363-365
dc.identifier.citationBrazilian Journal of Veterinary Research and Animal Science, v. 52, n. 4, p. 363-365, 2015.
dc.identifier.doi10.11606/issn.l6784456.v52i4p363-365
dc.identifier.issn1678-4456
dc.identifier.issn1413-9596
dc.identifier.scopus2-s2.0-84957863475
dc.identifier.urihttp://hdl.handle.net/11449/220559
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Veterinary Research and Animal Science
dc.sourceScopus
dc.subjectCanine distemper
dc.subjectDirect immunofluorescence
dc.subjectHnRT-PCR
dc.subjectKappa index
dc.titleAvaliação das técnicas de RT-PCR e heminested RT-PCR em cérebros de cães com sinais neurológicos compatíveis com cinomosept
dc.title.alternativeEvaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemperen
dc.typeArtigo

Arquivos

Coleções

Artigos