Smear layer removal and collagen fiber exposure using tetracycline hydrochloride conditioning

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dc.contributor.authorIshi, Eduardo de Paula [UNESP]
dc.contributor.authorDantas, Andréa Abi Rached [UNESP]
dc.contributor.authorBatista, Luiz Henrique Carvalho
dc.contributor.authorOnofre, Mirian Aparecida [UNESP]
dc.contributor.authorSmapaio, José Eduardo C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Alagoas (UFAL)
dc.date.accessioned2014-05-27T11:23:35Z
dc.date.available2014-05-27T11:23:35Z
dc.date.issued2008-07-01
dc.description.abstractAim: Smear layer removal and collagen fiber exposure may improve periodontal treatment and regeneration. This in vitro study assessed smear layer removal and collagen fiber exposure after tetracycline hydrochloride (TTC) application on root surfaces using scanning electron microscopy (SEM). Methods and Materials: Root cementum was removed with diamond burs followed by scaling and root planning. Four hundred fifty samples were divided into ten groups: a control (saline application) and nine different TTC concentrations were applied at doses of 10, 25, 50, 75, 100, 125, 150, 200, and 250 mg/ml. The TTC application was performed in all groups in three different ways (passive, brushing, and burnishing) and at three different periods of conditioning (1, 2, and 3 minutes). A previously trained, calibrated, and blind examiner evaluated photomicrographs of the samples using Sampaio's index (2005). Statistical analysis was performed using the Kruskal-Wallis' and Dunn's tests. Results: The concentrations of 50 mg/mL and 75 mg/mL applied by burnishing were the most effective in smear layer removal and collagen fiber exposure. Both the passive mode of application (p=0.0001) and 1 minute period of application (p=0.002) were the least effective. Conclusions: The concentrations of 50 mg/mL and 75 mg/mL applied by burnishing during 2 or 3 minutes were the most effective. Clinical Significance: These parameters may be applied in periodontal procedures involving TTC root conditioning to optimize results.en
dc.description.affiliationDepartment of Diagnostic and Surgery School of Dentistry at Araraquara São Paulo State University, UNESP, São Paulo
dc.description.affiliationElectrochemal Group Federal University of Alagoas, UFAL, Alagoas
dc.description.affiliationUnespDepartment of Diagnostic and Surgery School of Dentistry at Araraquara São Paulo State University, UNESP, São Paulo
dc.format.extent025-033
dc.identifierhttp://www.jaypeejournals.com/eJournals/ShowText.aspx?ID=1895&Type=FREE&TYP=TOP&IN=_eJournals/images/JPLOGO.gif&IID=161&isPDF=YES
dc.identifier.citationJournal of Contemporary Dental Practice, v. 9, n. 5, p. 025-033, 2008.
dc.identifier.issn1526-3711
dc.identifier.lattes0944020161060502
dc.identifier.scopus2-s2.0-46749131983
dc.identifier.urihttp://hdl.handle.net/11449/70466
dc.language.isoeng
dc.relation.ispartofJournal of Contemporary Dental Practice
dc.relation.ispartofsjr0,263
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectPeriodontics
dc.subjectSEM
dc.subjectSmear layer
dc.subjectTetracycline hydrochloride
dc.subjectantiinfective agent
dc.subjectfibrillar collagen
dc.subjecttetracycline
dc.subjectcementum
dc.subjectdental surgery
dc.subjectdose response
dc.subjectdrug effect
dc.subjecthistology
dc.subjecthuman
dc.subjectmethodology
dc.subjectpreventive dentistry
dc.subjectscanning electron microscopy
dc.subjectultrastructure
dc.subjectAnti-Bacterial Agents
dc.subjectDecalcification Technique
dc.subjectDental Cementum
dc.subjectDose-Response Relationship, Drug
dc.subjectFibrillar Collagens
dc.subjectHumans
dc.subjectMicroscopy, Electron, Scanning
dc.subjectRoot Planing
dc.subjectSmear Layer
dc.subjectTetracycline
dc.titleSmear layer removal and collagen fiber exposure using tetracycline hydrochloride conditioningen
dc.typeArtigo
unesp.author.lattes0944020161060502
unesp.author.lattes7460825090327014[2]
unesp.author.orcid0000-0002-8309-8837[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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