Antimicrobial activity of noncytotoxic concentrations of Salvia officinalis extract against bacterial and fungal species from the oral cavity

Página do item simplificado
dc.contributor.authorDe Oliveira, Jonatas Rafael [UNESP]
dc.contributor.authorDas Graças Figueiredo Vilela, Polyana [UNESP]
dc.contributor.authorDe Aguiar Almeida, Rosilene Batista [UNESP]
dc.contributor.authorDe Oliveira, Felipe Eduardo [UNESP]
dc.contributor.authorTalge Carvalho, Cláudio Antonio
dc.contributor.authorAfonso Camargo, Samira Esteves
dc.contributor.authorCardoso Jorge, Antonio Olavo [UNESP]
dc.contributor.authorDe Oliveira, Luciane Dias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCollege of Dentistry
dc.date.accessioned2019-10-06T17:00:36Z
dc.date.available2019-10-06T17:00:36Z
dc.date.issued2019-01-01
dc.description.abstractThe use of medicinal plants can be an alternative method for the control of microorganisms responsible for human infections. This study evaluated the antimicrobial activity of Salvia officinalis Linnaeus (sage) extract on clinical samples isolated from the oral cavity and reference strains of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata. In addition, testing assessed the cytotoxic effect of S officinalis on murine macrophages (RAW 264.7). Minimum inhibitory, minimum bactericidal, and minimum fungicidal concentrations of S officinalis extract were determined by broth microdilution method in 60 microbial samples. The cytotoxicity was checked by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The quantities of the proinflammatory cytokines interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) produced by RAW 264.7 were analyzed by an enzyme-linked immunosorbent assay. An S officinalis concentration of 50.0 mg/mL was effective against all microorganisms. Regarding cytotoxicity, the groups treated with 50.0-, 25.0-, and 12.5-mg/mL concentrations of S officinalis presented cell viability statistically similar to that of the control group, which was 100% viable. The production of IL-1β and TNF-α was inhibited at a 50.0-mg/mL concentration of S officinalis. Thus, S officinalis extract presented antimicrobial activity on all isolates of Staphylococcus spp, S mutans, and Candida spp. No cytotoxic effect was observed, as demonstrated by the survival of RAW 264.7 and inhibition of IL-1β and of TNF-α.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis São Paulo State University (UNESP) Institute of Science and Technology
dc.description.affiliationDepartment of Restorative Dentistry University of Florida College of Dentistry
dc.description.affiliationDepartment of Restorative Dental Sciences University of Florida College of Dentistry
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis São Paulo State University (UNESP) Institute of Science and Technology
dc.format.extent22-26
dc.identifier.citationGeneral Dentistry, v. 67, n. 1, p. 22-26, 2019.
dc.identifier.issn0363-6771
dc.identifier.scopus2-s2.0-85060058093
dc.identifier.urihttp://hdl.handle.net/11449/190048
dc.language.isoeng
dc.relation.ispartofGeneral Dentistry
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCandida spp
dc.subjectCytotoxicity
dc.subjectSalvia officinalis
dc.subjectStaphylococcus spp
dc.subjectStreptococcus mutans
dc.titleAntimicrobial activity of noncytotoxic concentrations of Salvia officinalis extract against bacterial and fungal species from the oral cavityen
dc.typeArtigo
unesp.author.lattes0053567153623569[7]
unesp.author.orcid0000-0002-1747-6158[7]

Arquivos

Coleções

Artigos - Biociências e Diagnóstico Bucal - ICT