Silver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesion

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dc.contributor.authorMendes-Gouvêa, Carla Corrêa [UNESP]
dc.contributor.authorDanelon, Marcelle [UNESP]
dc.contributor.authorVieira, Ana Paula Miranda [UNESP]
dc.contributor.authordo Amaral, Jackeline Gallo [UNESP]
dc.contributor.authorde Souza Neto, Francisco Nunes [UNESP]
dc.contributor.authorGorup, Luiz Fernando
dc.contributor.authorCamargo, Emerson Rodrigues
dc.contributor.authorDelbem, Alberto Carlos Botazzo [UNESP]
dc.contributor.authorBarbosa, Debora Barros [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Ribeirão Preto - UNAERP
dc.contributor.institutionFederal University of Alfenas
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2023-03-02T11:50:58Z
dc.date.available2023-03-02T11:50:58Z
dc.date.issued2022-10-01
dc.description.abstractObjectives: The aim of this study were to produce a multifunctional nanocomposite combining silver nanoaparticles (Ag), sodium trimetaphosphate (TMP) and fluoride (F), to investigate its effect on dental enamel demineralization and on biofilms of Streptococcus mutans and Candida albicans. Methods: Bovine enamel blocks were submitted to five pH cycles and treated 2x/day with 100 ppm F, 225 ppm F, 100 ppm F + 0.2%TMP or 100 ppm F + 0.2%TMP+10% Ag (100F/TMP/Ag). Next, surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), enamel fluoride (F) and calcium (Ca) concentration were determined. Biofilms from single and dual species of S. mutans and C. albicans were treated with 100F/TMP/Ag, Ag or chlorhexidine gluconate for 24 h. The antibiofilm effect was evaluated by colony-forming unit counting and Scanning Electron Microscopy. Results: The nanocomposite reduced 43.0% of %SH and was similar with samples treated with 225F, 100F/TMP and 100/TMP/Ag. The attribute of F and/or TMP in reducing ΔKHN in 5–20 μm was not affected by the addiction of Ag (110F = 225F = 100F/TMP = 100F/TMP/Ag > Negative Control). Further, 100F/TMP/Ag strongly reduced viable cells of S. mutans in dual biofilms (∼5 log10cm2) and structurally affected the biofilms. Conclusion: The 100F/TMP/F promoted a protective effect against enamel demineralization and was able to significantly inhibit the growth of biofilms of S. mutans and C. albicans. Clinical significance: The focus on prevention and non-invasive dental treatment is the most effective and least costly way to improve the population's oral health conditions. We present a nanocomposite for a multiple approach in prevention of caries.en
dc.description.affiliationGraduate Program of Dental Science School of Dentistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationSchool of Dentistry University of Ribeirão Preto - UNAERP, São Paulo
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationInstitute of Chemistry Federal University of Alfenas, Minas Gerais
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos, São Paulo
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespGraduate Program of Dental Science School of Dentistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), São Paulo
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), São Paulo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 001
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2022.104245
dc.identifier.citationJournal of Dentistry, v. 125.
dc.identifier.doi10.1016/j.jdent.2022.104245
dc.identifier.issn0300-5712
dc.identifier.scopus2-s2.0-85136712235
dc.identifier.urihttp://hdl.handle.net/11449/242203
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.sourceScopus
dc.subjectDental caries
dc.subjectFluoride
dc.subjectNanoparticles
dc.subjectPolyphosphates
dc.subjectSilver Compounds
dc.titleSilver nanoparticles associated with a polyphosphate and fluoride enhance the prevention of enamel demineralization and impact on dual-biofilm adhesionen
dc.typeArtigo
unesp.author.orcid0000-0003-2091-649X 0000-0003-2091-649X[2]
unesp.author.orcid0000-0002-2800-5490[3]
unesp.author.orcid0000-0002-8899-4390[5]
unesp.author.orcid0000-0003-2646-8026[6]
unesp.author.orcid0000-0003-2893-5353[7]
unesp.author.orcid0000-0002-8159-4853[8]
unesp.author.orcid0000-0002-3623-5093[9]

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