Dextran sulfate/Triton X two-phase micellar systems as an alternative first purification step for clavulanic acid

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dc.contributor.authorSilva, Marcela de Siqueira Cardoso
dc.contributor.authorEbinuma, Valéria de Carvalho Santos [UNESP]
dc.contributor.authorLopes, André Moreni
dc.contributor.authorRangel-Yagui, Carlota de Oliveira
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-01-28T16:55:57Z
dc.date.available2016-01-28T16:55:57Z
dc.date.issued2015
dc.description.abstractThe development of efficient and cost-effective downstream processes is essential for any pharmaceutical products. Clavulanic acid (CA) is a ß-lactamic drug used combined with antibiotics to inhibit bacterial resistance mediated by ß-lactamase enzymes. CA industrial purification involves techniques such as liquid–liquid extraction with organic solvents and chromatographic techniques, resulting in low recovery yields. In this way, techniques more suitable for biomolecules such as liquid–liquid extraction based on aqueous two-phase systems, which contains 60–90% of water, are of great interest. In this work, aqueous two-phase micellar systems (ATPMS) formed by the nonionic surfactants Triton X-114 and Triton X-100 in the presence or absence of dextran sulfate (Dx-S) were investigated as a first step of CA purification. The drug was found to be stable in the presence of polymers and surfactants with mass balances higher than 80% for all the conditions evaluated. CA partitioning in ATPMS was investigated in the presence of concentrations of Dx-S lower than 4%, CA partitioned more to micelle-poor phase. However, increasing the polymer concentration (8 and 14%), we observed a phase inversion with both surfactants and the drug partitioned more pronounced to the micelle rich-phase, with partition coefficients up to 1.83 with TX-100. The results show that Dx-S/Triton X systems can be used as a first step of CA extraction and the conditions can be adjusted depending on the subsequent extraction methods.en
dc.description.affiliationDepartment of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Brazil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Department of Bioprocess and Biotechnology, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Faculdade de Araraquara - Ciências Farmacêuticas, Centro, CEP 14801902, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Bioprocessos e Biotecnologia, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Faculdade de Araraquara - Ciências Farmacêuticas, Centro, CEP 14801902, SP, Brasil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação Amparo à Pesquisa Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 290076/2011-0
dc.description.sponsorshipIdCNPq: 305659/2011-1
dc.description.sponsorshipIdFAPESP: 2010/02324-0
dc.description.sponsorshipIdFAPESP: 2011/18088-6
dc.format.extent80-86
dc.identifierhttp://dx.doi.org/10.1016/j.fluid.2015.04.026
dc.identifier.citationFluid Phase Equilibria, v. 399, p. 80-86, 2015.
dc.identifier.doi10.1016/j.fluid.2015.04.026
dc.identifier.issn0378-3812
dc.identifier.lattes9913041375494177
dc.identifier.urihttp://hdl.handle.net/11449/133639
dc.language.isoeng
dc.relation.ispartofFluid Phase Equilibria
dc.relation.ispartofjcr2.197
dc.relation.ispartofsjr0,950
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectAntibioticsen
dc.subjectClavulanic aciden
dc.subjectLiquid–liquid extractionen
dc.subjectPurification processen
dc.subjectAqueous two-phase micellar systemsen
dc.subjectDextran sulfateen
dc.titleDextran sulfate/Triton X two-phase micellar systems as an alternative first purification step for clavulanic aciden
dc.typeArtigo
unesp.author.lattes9913041375494177
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentBioprocessos e Biotecnologiapt

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