Role of catecholaminergic neurones of the caudal ventrolateral medulla in cardiovascular responses induced by acute changes in circulating volume in rats

Página do item simplificado
dc.contributor.authorPedrino, Gustavo Rodrigues
dc.contributor.authorMaurino, Isabela
dc.contributor.authorde Almeida Colombari, Debora Simoes
dc.contributor.authorCravo, Sergio Luiz
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:45:43Z
dc.date.available2014-05-20T13:45:43Z
dc.date.issued2006-11-01
dc.description.abstractSeveral findings suggest that catecholaminergic neurones in the caudal ventrolateral medulla (CVLM) contribute to body fluid homeostasis and cardiovascular regulation. The present study sought to determine the effects of lesions of these neurones on the cardiovascular responses induced by changes in circulating volume. All experiments were performed in male Wistar rats (320-360 g). Medullary catecholaminergic neurones were lesioned by microinjection of anti-dopamine beta-hydroxylase-saporin (6.3 ng in 60 nl; SAP rats, n = 14) into the CVLM, whereas sham rats received microinjections of free saporin (1.3 ng in 60 nl, n = 15). Two weeks later, rats were anaesthetized (urethane, 1.2 g kg(-1), I.V..), instrumented for measurement of mean arterial pressure (MAP), renal blood flow (RBF) and renal vascular conductance (RVC), and infused with hypertonic saline (HS; 3 M NaCl, 0.18 ml (100 g body weight)(-1), I.V.) or an isotonic solution (volume expansion, VE; 4% Ficoll, 1% of body weight, I.V.). In sham rats, HS induced sustained increases in RBF and RVC (155 +/- 7 and 145 +/- 6% of baseline, at 20 min after HS). In SAP rats, RBF responses to HS were blunted (125 +/- 6%) and RVC increases were abolished (108 +/- 5%) 20 min after HS. Isotonic solution increased RBF and RVC in sham rats (149 +/- 10 and 145 +/- 12% of baseline, respectively, at 20 min). These responses were reduced in SAP rats (131 +/- 6 and 126 +/- 5%, respectively, at 20 min). Pressor responses to HS were larger in SAP rats than in sham rats (17 +/- 5 versus 9 +/- 2 mmHg, at 20 min), whereas during VE these responses were similar in both groups (6 +/- 3 versus 4 +/- 6 mmHg, at 20 min). Immunohistochemical analysis indicates that microinjections of anti-D beta H-saporin produced extensive destruction within the A1/C1 cell groups in the CVLM. These results suggest that catecholaminergic neurones mediate the cardiovascular responses to VE or increases in plasma sodium levels.en
dc.description.affiliationUniv Fed São Paulo, Dept Physiol, Escola Paulista Med, BR-04023060 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Physiol & Pathol, Fac Odontol, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Physiol & Pathol, Fac Odontol, Araraquara, SP, Brazil
dc.format.extent995-1005
dc.identifierhttp://dx.doi.org/10.1113/expphysiol.2006.034611
dc.identifier.citationExperimental Physiology. Oxford: Blackwell Publishing, v. 91, n. 6, p. 995-1005, 2006.
dc.identifier.doi10.1113/expphysiol.2006.034611
dc.identifier.issn0958-0670
dc.identifier.urihttp://hdl.handle.net/11449/16104
dc.identifier.wosWOS:000241735200006
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofExperimental Physiology
dc.relation.ispartofjcr2.732
dc.relation.ispartofsjr1,238
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleRole of catecholaminergic neurones of the caudal ventrolateral medulla in cardiovascular responses induced by acute changes in circulating volume in ratsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
unesp.author.orcid0000-0003-4331-0271[3]
unesp.author.orcid0000-0002-3441-5576[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Fisiologia e Patologia - FOAR