Effects of uracil calculi on cell growth and apoptosis in the BBN- initiated Wistar rat urinary bladder mucosa

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dc.contributor.authorOliveira, Susie Vieira de [UNESP]
dc.contributor.authorCamargo, João Lauro Viana de [UNESP]
dc.contributor.authorCardoso, Paulo Roberto [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorFukushima, Shoji
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionOsaka City University
dc.date.accessioned2014-05-27T11:19:45Z
dc.date.available2014-05-27T11:19:45Z
dc.date.issued1999-07-28
dc.description.abstractThe different potential of initiated and non-initiated urinary bladder mucosa (UBM) to develop neoplasia was quantitatively evaluated in the male Wistar rat. Initiation of carcinogenesis was accomplished with N-butyl-N-(4- hydroxybutyl)-nitrosamine (BBN). Stimuli for cell proliferation and apoptosis were obtained by exposure followed by withdrawal of 3% Uracil in the diet. The proliferation index (PI) was estimated in UBM immunostained for the proliferating nuclear cell antigen (PCNA). The apoptotic index (AI) and the density of papillary/nodular hyperplasia (PNH) were estimated in hematoxilin- eosin stained sections. PNH was the main proliferative response to the mechanical irritation by uracil, irrespective of previous initiation with BBN. Uracil exposure induced higher PI and PNH density in the initiated rats. After uracil withdrawal, there was a significant increase of the AI in both uracil-treated groups, which correlated well to the respective PNH density. However, at the end of the experiment, PNH incidence and density were significantly higher in the BBN-initiated mucosa, which also presented 18% incidence of papillomas and 27% of carcinomas. Therefore, under prolonged uracil calculi trauma, the UBM of BBN-initiated Wistar rats gives rise to epithelial proliferative lesions that progress to neoplasia through acquired resistance to apoptosis.en
dc.description.affiliationDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationDepto. de Bioestatística Instituto de Biociências UNESP, Botucatu, SP
dc.description.affiliationOsaka City University Medical School Department of Pathology, Osaka
dc.description.affiliationDepartamento de Patologia Faculdade de Medicina Univ. Estadual Paulista (UNESP), CEP 18.618-000 Botucatu, SP
dc.description.affiliationUnespDepartamento de Patologia Faculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationUnespDepto. de Bioestatística Instituto de Biociências UNESP, Botucatu, SP
dc.description.affiliationUnespDepartamento de Patologia Faculdade de Medicina Univ. Estadual Paulista (UNESP), CEP 18.618-000 Botucatu, SP
dc.format.extent293-303
dc.identifierhttp://dx.doi.org/10.1002/(SICI)1520-6866(1999)19:4<293::AID-TCM6>3.0.CO;2-#
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291520-6866%281999%2919:4%3C293::AID-TCM6%3E3.0.CO;2-%23/abstract
dc.identifier.citationTeratogenesis Carcinogenesis and Mutagenesis, v. 19, n. 4, p. 293-303, 1999.
dc.identifier.doi10.1002/(SICI)1520-6866(1999)19:4<293::AID-TCM6>3.0.CO;2-#
dc.identifier.issn0270-3211
dc.identifier.lattes8727897080522289
dc.identifier.scopus2-s2.0-0032788208
dc.identifier.urihttp://hdl.handle.net/11449/65811
dc.language.isoeng
dc.relation.ispartofTeratogenesis Carcinogenesis and Mutagenesis
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCell proliferation
dc.subjectTwo-step carcinogenesis
dc.subjectUrinary calculi
dc.subjectUrothelial neoplasia
dc.subjectWistar rat
dc.subjectcell antigen
dc.subjectn butyl n (4 hydroxybutyl)nitrosamine
dc.subjecturacil
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectapoptosis
dc.subjectbladder mucosa
dc.subjectcarcinogenesis
dc.subjectcell growth
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectimmunohistochemistry
dc.subjectpriority journal
dc.subjectrat
dc.subjectstone formation
dc.subjectApoptosis
dc.subjectButylhydroxybutylnitrosamine
dc.subjectCarcinogens
dc.subjectCell Division
dc.subjectHyperplasia
dc.subjectMucous Membrane
dc.subjectPapilloma
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectRats
dc.subjectUracil
dc.subjectUrinary Bladder
dc.subjectUrinary Bladder Calculi
dc.subjectUrinary Bladder Neoplasms
dc.subjectAnimalia
dc.subjectRattus norvegicus
dc.titleEffects of uracil calculi on cell growth and apoptosis in the BBN- initiated Wistar rat urinary bladder mucosaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes8727897080522289[4]
unesp.author.orcid0000-0002-7719-9682[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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