Immunohistochemical expression of HLA-DR in the conjunctiva of patients under topical prostaglandin analogs treatment

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dc.contributor.authorRodrigues, Maria de Lourdes Veronese
dc.contributor.authorFelipe Crosta, Daniela Pereira Da Silva
dc.contributor.authorSoares, Christiane Pienna [UNESP]
dc.contributor.authorDeghaide, Neifi Hassam Saloum
dc.contributor.authorDuarte, Roberta [UNESP]
dc.contributor.authorSakamoto, Fabiano S.
dc.contributor.authorFurtado, João Marcello Fortes
dc.contributor.authorPaula, Jayter Silva
dc.contributor.authorDonadi, Eduardo Antonio
dc.contributor.authorSoares, Edson Garcia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:23:52Z
dc.date.available2014-05-27T11:23:52Z
dc.date.issued2009-03-01
dc.description.abstractPURPOSE: Subclinical inflammation may be observed in patients using topical antiglaucomatous drugs. The objective of this study was to investigate inflammation in conjunctiva of glaucoma patients using prostaglandin analogs, by the detection of an immunogenetic marker (HLA-DR) and compare the effect of 3 different drugs: latanoprost, bimatoprost, and travoprost in the induction of this inflammation. SUBJECTS AND METHODS: Thirty-three patients with primary open-angle glaucoma were evaluated without and with prostaglandin analogs topical therapy. Imprints of conjunctival cells were obtained, fixed on glass slides, and prepared for immunohistochemical analysis. RESULTS: Before the use of prostaglandin analogs, 4 of the 33 patients evaluated presented expression of HLA-DR in the conjunctiva (mild). After 1 month on prostaglandin analog treatment, all but 1 patient presented HLA-DR staining. HLA-DR expression of these 32 patients was scored as mild (19 patients), medium (11 patients), or intense (2 patients). The differences were statistically significant both when the presence and the increased expression of HLA-DR were considered (P<0.001). When the 3 different groups were analyzed (latanoprost, bimatoprost, and travoprost) no statistically significant difference was found (P=0.27). CONCLUSIONS: The use of prostaglandin analogs eye drops provokes a subclinical inflammatory reaction, observed by HLA-DR expression, even after a short period of treatment, independently of the class of the prostaglandin analogs used. © 2009 Lippincott Williams & Wilkins, Inc.en
dc.description.affiliationDepartments of Ophthalmology, Internal Medicine and Pathology Medical School of Ribeirão Preto University of São Paulo
dc.description.affiliationDepartment of Cytology and Cell Biology School of Pharmaceutical Sciences State University of São Paulo (UNESP)
dc.description.affiliation, Av. Bandeirantes 3900, Ribeirão Preto 14048-900, SP
dc.description.affiliationUnespDepartment of Cytology and Cell Biology School of Pharmaceutical Sciences State University of São Paulo (UNESP)
dc.format.extent197-200
dc.identifierhttp://dx.doi.org/10.1097/IJG.0b013e31818153f4
dc.identifier.citationJournal of Glaucoma, v. 18, n. 3, p. 197-200, 2009.
dc.identifier.doi10.1097/IJG.0b013e31818153f4
dc.identifier.issn1057-0829
dc.identifier.lattes1768025290373669
dc.identifier.orcid0000-0003-1740-7360
dc.identifier.scopus2-s2.0-65649136930
dc.identifier.urihttp://hdl.handle.net/11449/70941
dc.language.isoeng
dc.relation.ispartofJournal of Glaucoma
dc.relation.ispartofjcr1.742
dc.relation.ispartofsjr1,080
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectConjunctival in.ammation
dc.subjectGlaucoma treatment
dc.subjectHLA-DR
dc.subjectProstaglandin analog
dc.subjectbimatoprost
dc.subjectHLA DR antigen
dc.subjectlatanoprost
dc.subjecttravoprost
dc.subjectamide
dc.subjectantihypertensive agent
dc.subjectbiological marker
dc.subjectcloprostenol
dc.subjectdrug derivative
dc.subjectprostaglandin F
dc.subjectadult
dc.subjectaged
dc.subjectantigen expression
dc.subjectclinical article
dc.subjectconjunctiva
dc.subjectconjunctivitis
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunohistochemistry
dc.subjectmale
dc.subjectopen angle glaucoma
dc.subjectpriority journal
dc.subjectchemically induced disorder
dc.subjectenzyme immunoassay
dc.subjectmetabolism
dc.subjectmiddle aged
dc.subjecttopical drug administration
dc.subjectAdministration, Topical
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectAmides
dc.subjectAntihypertensive Agents
dc.subjectBiological Markers
dc.subjectCloprostenol
dc.subjectConjunctivitis
dc.subjectFemale
dc.subjectGlaucoma, Open-Angle
dc.subjectHLA-DR Antigens
dc.subjectHumans
dc.subjectImmunoenzyme Techniques
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectProstaglandins F, Synthetic
dc.titleImmunohistochemical expression of HLA-DR in the conjunctiva of patients under topical prostaglandin analogs treatmenten
dc.typeArtigo
dcterms.licensehttp://edmgr.ovid.com/spine/accounts/copyrightTransfer.pdf
unesp.author.lattes1768025290373669[3]
unesp.author.orcid0000-0003-1740-7360[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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