Molecular characterization and chromosomal localization of two families of satellite DNA in Prochilodus lineatus (Pisces, Prochilodontidae), a species with B chromosomes

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dc.contributor.authorDe Jesus, Célia Maria
dc.contributor.authorGaletti Jr., Pedro M.
dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.authorMoreira-Filho, Orlando
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:39Z
dc.date.available2014-05-27T11:20:39Z
dc.date.issued2003-05-01
dc.description.abstractProchilodus lineatus, an abundant species in the Mogi-Guaçu river basin, represents a large part of the region's fishing potential. Karyotypic analyses based on classic cytogenetic techniques have revealed the presence of 54 metasubmetacentric type chromosomes, together with the occurrence of small supernumerary chromosomes with intra and interindividual variations. This paper describes the genomic organization of two families of satellite DNA in the P. lineatus genome. The chromosomal localization these two repetitive DNA families through fluorescence in situ hybridization (FISH) demonstrated that the SATH1 satellite DNA family, composed of approximately 900 bp, was located in the pericentromeric region of a group of chromosomes of the standard complement, as well as on all the B chromosomes. The SATH2 satellite family has a monomeric unit of 441 bp and was located in the pericentromeric regions of some chromosomes of the standard complement, but was absent in the B chromosomes. Double FISH analyses showed that these two families participate jointly in the pericentromeric organization of several chromosomes of this species. The data obtained in this study support the hypothesis that the B chromosomes derive from chromosomes of the standard complement, which are carriers of the SATH1 satellite DNA.en
dc.description.affiliationDepto. de Genet. e Evolucao Univ. Federal de São Carlos, Via. Washington Luiz km 235, CEP 13565-905 São Carlos, SP
dc.description.affiliationDepto. de Cie. Biológicas Faculdade de Cie. Farmaceuticas Universidade Estadual Paulista, 14801-902 Araraquara, SP
dc.description.affiliationUnespDepto. de Cie. Biológicas Faculdade de Cie. Farmaceuticas Universidade Estadual Paulista, 14801-902 Araraquara, SP
dc.format.extent25-32
dc.identifierhttp://dx.doi.org/10.1023/A:1022986816648
dc.identifier.citationGenetica, v. 118, n. 1, p. 25-32, 2003.
dc.identifier.doi10.1023/A:1022986816648
dc.identifier.issn0016-6707
dc.identifier.lattes5333250355049814
dc.identifier.scopus2-s2.0-0037405611
dc.identifier.urihttp://hdl.handle.net/11449/67269
dc.identifier.wosWOS:000181764800004
dc.language.isoeng
dc.relation.ispartofGenetica
dc.relation.ispartofjcr1.366
dc.relation.ispartofsjr0,649
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectB chromosome
dc.subjectFISH
dc.subjectPisces
dc.subjectProchilodus lineatus
dc.subjectSatellite DNA
dc.subjectAnimals
dc.subjectBlotting, Southern
dc.subjectBrazil
dc.subjectChromosome Mapping
dc.subjectDNA, Satellite
dc.subjectFishes
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectSequence Alignment
dc.subjectProchilodontidae
dc.titleMolecular characterization and chromosomal localization of two families of satellite DNA in Prochilodus lineatus (Pisces, Prochilodontidae), a species with B chromosomesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes5333250355049814
unesp.author.orcid0000-0001-5916-6126[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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