Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

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dc.contributor.authorMatos, Adaias Oliveira
dc.contributor.authorde Almeida, Amanda Bandeira
dc.contributor.authorBeline, Thamara
dc.contributor.authorTonon, Caroline C.
dc.contributor.authorCasarin, Renato Corrêa Viana
dc.contributor.authorWindsor, Lester Jack
dc.contributor.authorDuarte, Simone
dc.contributor.authorNociti, Francisco Humberto
dc.contributor.authorRangel, Elidiane Cipriano [UNESP]
dc.contributor.authorGregory, Richard L.
dc.contributor.authorBarão, Valentim Adelino Ricardo
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionIndiana University School of Dentistry
dc.contributor.institutionSchool of Dentistry
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:46:54Z
dc.date.available2020-12-12T02:46:54Z
dc.date.issued2020-12-01
dc.description.abstractOur goal was to create bio-functional chlorhexidine (CHX)-doped thin films on commercially pure titanium (cpTi) discs using the glow discharge plasma approach. Different plasma deposition times (50, 35 and 20 min) were used to create bio-functional surfaces based on silicon films with CHX that were compared to the control groups [no CHX and bulk cpTi surface (machined)]. Physico-chemical and biological characterizations included: 1. Morphology, roughness, elemental chemical composition, film thickness, contact angle and surface free energy; 2. CHX-release rate; 3. Antibacterial effect on Streptococcus sanguinis biofilms at 24, 48 and 72 h; 4. Cytotoxicity and metabolic activity using fibroblasts cell culture (NIH-F3T3 cells) at 1, 2, 3 and 4 days; 5. Protein expression by NIH-F3T3 cells at 1, 2, 3 and 4 days; and 6. Co-culture assay of fibroblasts cells and S. sanguinis to assess live and dead cells on the confocal laser scanning microscopy, mitochondrial activity (XTT), membrane leakage (LDH release), and metabolic activity (WST-1 assay) at 1, 2 and 3 days of co-incubation. Data analysis showed that silicon films, with or without CHX coated cpTi discs, increased surface wettability and free energy (p < 0.05) without affecting surface roughness. CHX release was maintained over a 22-day period and resulted in a significant inhibition of biofilm growth (p < 0.05) at 48 and 72 h of biofilm formation for 50 min and 20 min of plasma deposition time groups, respectively. In general, CHX treatment did not significantly affect NIH-F3T3 cell viability (p > 0.05), whereas cell metabolism (MTT assay) was affected by CHX, with the 35 min of plasma deposition time group displaying the lowest values as compared to bulk cpTi (p < 0.05). Moreover, data analysis showed that films, with or without CHX, significantly affected the expression profile of inflammatory cytokines, including IL-4, IL-6, IL-17, IFN-y and TNF-α by NIH-F3T3 cells (p < 0.05). Co-culture demonstrated that CHX-doped film did not affect the metabolic activity, cytotoxicity and viability of fibroblasts cells (p > 0.05). Altogether, the findings of the current study support the conclusion that silicon films added with CHX can be successfully created on titanium discs and have the potential to affect bacterial growth and inflammatory markers without affecting cell viability/proliferation rates.en
dc.description.affiliationDepartment of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP)
dc.description.affiliationDepartment of Biomedical Sciences and Comprehensive Care Indiana University School of Dentistry
dc.description.affiliationDepartment of Cariology Operative Dentistry and Dental Public Health Indiana University Purdue University Indianapolis School of Dentistry
dc.description.affiliationLaboratory of Technological Plasmas (LaPTec) São Paulo State University (UNESP) Science and Technology Institute of Sorocaba (ICTS)
dc.description.affiliationUnespLaboratory of Technological Plasmas (LaPTec) São Paulo State University (UNESP) Science and Technology Institute of Sorocaba (ICTS)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 001
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2020.111289
dc.identifier.citationMaterials Science and Engineering C, v. 117.
dc.identifier.doi10.1016/j.msec.2020.111289
dc.identifier.issn1873-0191
dc.identifier.issn0928-4931
dc.identifier.scopus2-s2.0-85088894507
dc.identifier.urihttp://hdl.handle.net/11449/201985
dc.language.isoeng
dc.relation.ispartofMaterials Science and Engineering C
dc.sourceScopus
dc.subjectAntibacterial
dc.subjectBiocompatible
dc.subjectBiofilm
dc.subjectChlorhexidine
dc.subjectTitanium
dc.titleSynthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materialsen
dc.typeArtigo

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