Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil

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dc.contributor.authorMoraes, L. A. [UNESP]
dc.contributor.authorPavan, M. A. [UNESP]
dc.contributor.authorSakate, R. K. [UNESP]
dc.contributor.authorBlystad, D. R.
dc.contributor.authorSpetz, C.
dc.contributor.authorFloistad, E.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:19:18Z
dc.date.available2018-11-26T16:19:18Z
dc.date.issued2015-01-01
dc.description.abstractViruses are a major problem in orchid production systems, as they promote considerable damage to its commercial value, since they directly affect its aesthetic appeal as well as productivity. The Potexvirus Cymbidium mosaic virus (CymMV) and the Tobamovirus Odontoglossum ringspot virus (ORSV) are of great economic importance worldwide. A survey of the viruses present in 232 orchid samples belonging to different genera and collected from 12 Sao Paulo State cities was carried out. The diagnosis was made by direct ELISA test for the detection of CymMV, ORSV, TSWV (Tomato spotted wilt virus), and CymRSV (Cymbidium ringspot virus), as well as indirect ELISA for Potyvirus detection, using universal antiserum against this genus. The results were confirmed by RT-PCR, with specific oligonucleotides designed for the coat protein (CP) gene of the CymMV and ORSV, as well as for the detection of Orchid fleck virus (OFV). Of the total of samples tested, 153 were positive for CymMV (65.9%), and 40 were positive for ORSV (17.2%). CymRSV, TSWV, OFV or any Potyvirus were not found in the samples analyzed. In 90% of the samples in which ORSV was detected, CymMV was also present in mixed infections. Nucleotide identity of the CymMV sequenced isolates ranged between 91 and 92% with CymMV genbank accession numbers AY571289.1, AB541572.1 and AB197937.1, and the phylogenetic tree supposes that, so far, this virus has shown a low variability rate, in terms of CP gene. These results denote the CymMV importance in the orchid production systems in Sao Paulo State, as well as possible synergistic link between this virus and ORSV.en
dc.description.affiliationSao Paulo State Univ, Coll Agron Sci, Dept Vegetal Prod, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Coll Agron Sci, Dept Vegetal Prod, Botucatu, SP, Brazil
dc.format.extent55-61
dc.identifier.citationXiii International Symposium On Virus Diseases Of Ornamental Plants. Leuven 1: Int Soc Horticultural Science, v. 1072, p. 55-61, 2015.
dc.identifier.issn0567-7572
dc.identifier.lattes9659822855697685
dc.identifier.orcid0000-0001-7526-640X
dc.identifier.urihttp://hdl.handle.net/11449/161142
dc.identifier.wosWOS:000368416300005
dc.language.isoeng
dc.publisherInt Soc Horticultural Science
dc.relation.ispartofXiii International Symposium On Virus Diseases Of Ornamental Plants
dc.relation.ispartofsjr0,198
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdetection
dc.subjectsymptoms
dc.subjectOrchidaceae
dc.subjectCymbidium mosaic virus
dc.subjectOdontoglossum ringspot virus
dc.titlePreliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazilen
dc.typeArtigo
dcterms.rightsHolderInt Soc Horticultural Science
unesp.author.lattes9659822855697685[3]
unesp.author.orcid0000-0001-7526-640X[3]
unesp.departmentProdução e Melhoramento Vegetal - FCApt

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Botucatu - FCA - Faculdade de Ciências Agronômicas