A systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic bases

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dc.contributor.authorCalmon, Marilia de Freitas [UNESP]
dc.contributor.authorde Souza, Aryane Tofanello [UNESP]
dc.contributor.authorCandido, Natalia Maria [UNESP]
dc.contributor.authorBartolomeu Raposo, Maria Irene
dc.contributor.authorTaboga, Sebastiao [UNESP]
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorNery, Jose G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T14:01:04Z
dc.date.available2014-05-20T14:01:04Z
dc.date.issued2012-12-01
dc.description.abstractMagnetic iron oxide nanoparticles (magnetite) (MNPs) were prepared using different organic and inorganic bases. Strong inorganic base (KOH) and organic bases (NH4OH and 1,4-diazabicyclo[2.2.2]octane (DABCO)) were used in the syntheses of the MNPs. The MNPs were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FT-IR) and magnetization measurements. MNPs prepared with strong inorganic base yielded an average size of 100 nm, whereas the average size of the MNPs prepared with the organic bases was 150 nm. The main competitive phase for MNPs prepared with the strong inorganic and organic bases was maghemite; however, syntheses with KOH yielded a pure magnetite phase. The transfection study performed with the MNPs revealed that the highest transfection rate was obtained with the MNPs prepared with KOH (74%). The correlation between the magnetic parameters and the transfection ratio without transfection agents indicated that MNPs prepared with KOH were a better vector for possible applications of these MNPs in biomedicine. HeLa cells incubated with MNP-KOH at 10 mu g/mL for 24 and 48 h exhibited a decrease in population in comparison with the control cells and it was presumably related to the toxicity of the MNPs. However, the cells incubated with MNP-KOH at 50 and 100 mu g/mL presented a very small difference in the viability between the cell populations studied at 24 and 48 h. These data illustrate the viability of HeLa cells treated with MNP-KOH and suggest the potential use of these MNPs in biomedical applications. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, Dept Biol, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Phys, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Geosci, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Biol, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Phys, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipBraile BioMédica Industria, Comercio e Representacoes Limitada
dc.description.sponsorshipIdFAPESP: 05/54703-6
dc.description.sponsorshipIdFAPESP: 09/11203-4
dc.description.sponsorshipIdCNPq: 301596/2011-5
dc.format.extent177-184
dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2012.05.026
dc.identifier.citationColloids and Surfaces B-biointerfaces. Amsterdam: Elsevier B.V., v. 100, p. 177-184, 2012.
dc.identifier.doi10.1016/j.colsurfb.2012.05.026
dc.identifier.issn0927-7765
dc.identifier.lattes7991082362671212
dc.identifier.lattes9165601469436240
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.urihttp://hdl.handle.net/11449/21578
dc.identifier.wosWOS:000307683900025
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofColloids and Surfaces B: Biointerfaces
dc.relation.ispartofjcr3.997
dc.relation.ispartofsjr1,071
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectIron oxide nanoparticlesen
dc.subjectInorganic baseen
dc.subjectOrganic basesen
dc.subjectHeLa cellsen
dc.subjectTransfection efficiencyen
dc.subjectToxicityen
dc.titleA systematic study of transfection efficiency and cytotoxicity in HeLa cells using iron oxide nanoparticles prepared with organic and inorganic basesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes7991082362671212[6]
unesp.author.lattes9165601469436240
unesp.author.orcid0000-0002-0970-4288[5]
unesp.author.orcid0000-0001-5693-6148[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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