Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: A randomized controlled trial

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dc.contributor.authorCarvalho, Armando de Mattos [UNESP]
dc.contributor.authorBadial, Peres Ramos [UNESP]
dc.contributor.authorÁlvarez, Luis Emiliano Cisneros [UNESP]
dc.contributor.authorYamada, Ana Lucia Miluzzi [UNESP]
dc.contributor.authorBorges, Alexandre Secorun [UNESP]
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authorHussni, Carlos Alberto [UNESP]
dc.contributor.authorAlves, Ana Liz Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:03Z
dc.date.available2014-05-27T11:30:03Z
dc.date.issued2013-07-25
dc.description.abstractIntroduction. Tendon injury is a major cause of lameness and decreased performance in athletic equines. Various therapies for tendonitis have been described; however, none of these therapies results in complete tissue regeneration, and the injury recurrence rate is high even after long recovery periods involving rest and physiotherapy. Methods. A lesion was induced with collagenase gel in the superficial digital flexor tendon in the center portion of the metacarpal region of eight equines of mixed breed. After two weeks, the lesions of the animals in the treated and control groups were treated through the intralesional administration of mesenchymal stem cells derived from adipose tissue (adMSCs) suspended in platelet concentrate (PC) and with phosphate buffered saline (PBS), respectively. Serial ultrasound analyses were performed every two weeks. After 16 weeks of therapy, a biopsy was performed for histopathological, immunohistochemical and gene expression (type I collagen (COL1A1), type III collagen (COL3A1), tenascin-C (TNC), tenomodulin (TNMD), and scleraxis (SCX)) analyses. Results: Differences in the ultrasound and histopathological analyses were observed between the groups. Improved results were reported in the group treated with adMSCs suspended in PC. There was no difference in the gene expression levels observed after the different treatments. The main results observed from the histopathological evaluation of the treated group were as follows: a prevention of the progression of the lesion, a greater organization of collagen fibers, and a decreased inflammatory infiltrate. A lack of progression of the lesion area and its percentage was observed in the ultrasound image, and increased blood flow was measured by Power Doppler. Conclusions: The use of adMSCs combined with PC for the therapy of experimentally induced tendonitis prevented the progression of the tendon lesion, as observed in the ultrasound examination, and resulted in a greater organization and decreased inflammation, as observed in the histopathological evaluation. These data demonstrate the therapeutic potential of this therapy for the treatment of equine tendonitis. © 2013 Carvalho et al.; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationBlood Center Botucatu Medical School São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationUnespDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationUnespDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.description.affiliationUnespBlood Center Botucatu Medical School São Paulo State University Rubião Junior, 18618-970 Botucatu, São Paulo
dc.identifierhttp://dx.doi.org/10.1186/scrt236
dc.identifier.citationStem Cell Research and Therapy, v. 4, n. 4, 2013.
dc.identifier.doi10.1186/scrt236
dc.identifier.file2-s2.0-84880335347.pdf
dc.identifier.issn1757-6512
dc.identifier.lattes9643433706163946
dc.identifier.lattes6020984937849801
dc.identifier.lattes7773733250141398
dc.identifier.scopus2-s2.0-84880335347
dc.identifier.urihttp://hdl.handle.net/11449/76051
dc.identifier.wosWOS:000323174400001
dc.language.isoeng
dc.relation.ispartofStem Cell Research and Therapy
dc.relation.ispartofjcr4.963
dc.relation.ispartofsjr1,685
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectHorse
dc.subjectStem cell
dc.subjectTendon lesion
dc.subjectTreatment
dc.subjectcollagen fiber
dc.subjectcollagen type 1
dc.subjectmembrane protein
dc.subjectphosphate buffered saline
dc.subjectscleraxis
dc.subjecttenascin
dc.subjecttenomodulin
dc.subjectunclassified drug
dc.subjectadipose tissue
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectautologous stem cell transplantation
dc.subjectblood flow
dc.subjectcell isolation
dc.subjectclinical evaluation
dc.subjectCOL1A1 gene
dc.subjectCOL3A1 gene
dc.subjectcontrolled study
dc.subjectdigital flxeor tendon therapy
dc.subjectDoppler flowmeter
dc.subjectequine tendonitis
dc.subjectfemale
dc.subjectgene
dc.subjectgene expression
dc.subjecthistopathology
dc.subjecthorse disease
dc.subjectimage analysis
dc.subjectimmunohistochemistry
dc.subjectinflammatory infiltrate
dc.subjectmale
dc.subjectmesenchymal stem cell transplantation
dc.subjectnonhuman
dc.subjectphysical activity
dc.subjectpriority journal
dc.subjectprophylaxis
dc.subjectrandomized controlled trial
dc.subjectSCX gene
dc.subjecttendinitis
dc.subjecttendon
dc.subjectthrombocyte transfusion
dc.subjecttnc gene
dc.subjectTNMD gene
dc.subjecttreatment outcome
dc.subjecttreatment response
dc.subjectultrasound
dc.subjectAnimalia
dc.subjectEquidae
dc.titleEquine tendonitis therapy using mesenchymal stem cells and platelet concentrates: A randomized controlled trialen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes9643433706163946[5]
unesp.author.lattes6020984937849801[7]
unesp.author.lattes7773733250141398[8]
unesp.author.orcid0000-0001-6256-8089[5]
unesp.author.orcid0000-0001-9092-7819[8]
unesp.author.orcid0000-0001-5421-2904[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ
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