Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time

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dc.contributor.authorSilva, Ricardo Henrique Alves da
dc.contributor.authorQuiezi, Rodrigo Gonçalves [UNESP]
dc.contributor.authorBertolacini, Claudia Danielli Pereira [UNESP]
dc.contributor.authorCarvalho, Suzana Papile Maciel
dc.contributor.authorGasque, Kellen Cristina da Silva
dc.contributor.authorSilva, Carina Thais de Almeida e
dc.contributor.authorBicudo, Lucilene Arilho Ribeiro
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-07-07T12:35:41Z
dc.date.available2016-07-07T12:35:41Z
dc.date.issued2012
dc.description.abstractIntroduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed.en
dc.description.affiliationUniversidade de São Paulo (USP), Faculdade de Odontologia de Ribeirão Preto (FORP), Departamento de Clínica Infantil e Odontologia Preventiva e Social, Ribeirão Preto, SP, Brasil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências (IBB), Departamento de Genética, Botucatu, SP, Brasil
dc.description.affiliationUniversidade de São Paulo (USP), Faculdade de Odontologia (FO), São Paulo, SP, Brasil
dc.description.affiliationUniversidade de São Paulo (USP), Faculdade de Odontologia de Bauru (FOB), Bauru, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências (IBB), Departamento de Genética, Botucatu, SP, Brasil
dc.format.extent67-73
dc.identifierhttp://www.univille.edu.br/pt-BR/departamentos/odontologia/revista-rsbo/edicoes--2012/RSBO---v9---n01/725482
dc.identifier.citationRSBO. Revista Sul-Brasileira de Odontologia, v. 9, n. 1, p. 67-73, 2012.
dc.identifier.fileISSN1806-7727-2012-09-01-67-73.pdf
dc.identifier.issn1806-7727
dc.identifier.urihttp://hdl.handle.net/11449/140847
dc.language.isoeng
dc.relation.ispartofRSBO. Revista Sul-Brasileira de Odontologia
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectForensic dentistryen
dc.subjectForensic anthropologyen
dc.subjectForensic sciencesen
dc.titleHuman identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure timeen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentGenéticapt

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