Determination of fluoroacetate and fluoride in blood serum by capillary zone electrophoresis using capacitively coupled contactless conductivity detection

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dc.contributor.authorRajh Vidal, Denis Tadeu
dc.contributor.authorAugelli, Marcio Antonio [UNESP]
dc.contributor.authorHotta, Guilherme Minoru
dc.contributor.authorLopes, Fernando Silva
dc.contributor.authordo Lago, Claudimir Lucio
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Nacl Ciência & Tecnol Bioanalit
dc.date.accessioned2014-05-20T13:27:48Z
dc.date.available2014-05-20T13:27:48Z
dc.date.issued2011-04-01
dc.description.abstractFluoroacetate is a highly toxic species naturally found in plants and in commercial products (compound 1080) for population control of several undesirable animal species. However, it is non-selective and toxic to many other animals including humans, and thus its detection is very important for forensic purposes. This paper presents a sensitive and fast method for the determination of fluoroacetate in blood serum using capillary electrophoresis with capacitively coupled contactless conductivity detection. Serum blood samples were treated with ethanol to remove proteins. The samples were analyzed in BGE containing 15 mmol/L histidine and 30 mmol/L gluconic acid (pH 3.85). The calibration curve was linear up to 75 mu mol/L (R(2) = 0.9995 for N = 12). The detection limit in the blood serum was 0.15 mg/kg, which is smaller than the lethal dose for humans and other animals. Fluoride, a metabolite of the fluoroacetate defluorination, could also be detected for levels greater than 20 mu mol/L, when polybrene was used for reversion of the EOF. CTAB and didecyldimethylammonium bromide are not useful for this task because of the severe reduction of the fluoride level. However, no interference was observed for fluoroacetate.en
dc.description.affiliationUniv São Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, São Paulo, Brazil
dc.description.affiliationInst Nacl Ciência & Tecnol Bioanalit, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent896-899
dc.identifierhttp://dx.doi.org/10.1002/elps.201000475
dc.identifier.citationElectrophoresis. Malden: Wiley-blackwell, v. 32, n. 8, p. 896-899, 2011.
dc.identifier.doi10.1002/elps.201000475
dc.identifier.issn0173-0835
dc.identifier.urihttp://hdl.handle.net/11449/9219
dc.identifier.wosWOS:000289352900015
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofElectrophoresis
dc.relation.ispartofjcr2.569
dc.relation.ispartofsjr0,745
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBlood serumen
dc.subjectCapacitively coupled contactless conductivity detectionen
dc.subjectCompound 1080en
dc.subjectFluorideen
dc.subjectFluoroacetateen
dc.titleDetermination of fluoroacetate and fluoride in blood serum by capillary zone electrophoresis using capacitively coupled contactless conductivity detectionen
dc.typeArtigo
dcterms.licensehttp://www.wiley-vch.de/publish/en/authors/auguidelines/
dcterms.rightsHolderWiley-blackwell
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Engenharia, Guaratinguetápt

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