Possible interspecific origin of the B chromosome of Hypsiboas albopunctatus (Spix, 1824) (Anura, Hylidae), revealed by microdissection, chromosome painting, and reverse hybridisation

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dc.contributor.authorGruber, Simone Lilian
dc.contributor.authorDiniz, Debora
dc.contributor.authorSobrinho-Scudeler, Patricia Elda [UNESP]
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.authorBaptista Haddad, Celio Fernando [UNESP]
dc.contributor.authorKasahara, Sanae
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUESB
dc.date.accessioned2015-03-18T15:56:22Z
dc.date.available2015-03-18T15:56:22Z
dc.date.issued2014-01-01
dc.description.abstractThe B chromosome in the hylid Hypsiboas albopunctatus (2n = 22 + B) is small, almost entirely composed of C-positive heterochromatin, and does not pair with any chromosome of the A complement. B probe, obtained by microdissection and DOP-PCR amplification, was used to search for homology between the B and regular chromosomes of H. albopunctatus and of the related species H. raniceps (Cope, 1862). Reverse hybridisation was also carried out in the investigation. The B probe exclusively painted the supernumerary, not hybridising any other chromosomes in H. albopunctatus, but all H. raniceps chromosomes showed small labelling signals. This result might be an indication that differences exist between the repetitive sequences of A and B chromosomes of H. albopunctatus, and that the chromosomes of H. raniceps and the heterochromatin of the B chromosome of H. albopunctatus are enriched with the same type of repetitive DNA. In meiotic preparations, the B labelled about 30% of scored spermatids, revealing a non-mendelian inheritance, and the painted B in micronucleus suggests that the supernumerary is eliminated from germ line cells. Although our results could suggest an interespecific origin of the B at first sight, further analysis on its repetitive sequences is still necessary. Nevertheless, the accumulation of repetitive sequences, detected in another species, even though closely related, remains an intriguing question.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUESB, Dept Ciencias Biol, BR-45206190 Jequie, BA, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Zool, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Zool, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent185-197
dc.identifierhttp://dx.doi.org/10.3897/CompCytogen.v8i3.7771
dc.identifier.citationComparative Cytogenetics. Sofia: Pensoft Publ, v. 8, n. 3, p. 185-197, 2014.
dc.identifier.doi10.3897/CompCytogen.v8i3.7771
dc.identifier.fileWOS000341621900002.pdf
dc.identifier.issn1993-0771
dc.identifier.lattes0458077399058762
dc.identifier.lattes8422327495725206
dc.identifier.urihttp://hdl.handle.net/11449/117533
dc.identifier.wosWOS:000341621900002
dc.language.isoeng
dc.publisherPensoft Publ
dc.relation.ispartofComparative Cytogenetics
dc.relation.ispartofjcr1.319
dc.relation.ispartofsjr0,431
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectSupernumerary chromosomeen
dc.subjectFISHen
dc.subjectGISHen
dc.subjectreplication bandingen
dc.subjectHylidae familyen
dc.subjectheterochromatinen
dc.subjectrepetitive DNAen
dc.titlePossible interspecific origin of the B chromosome of Hypsiboas albopunctatus (Spix, 1824) (Anura, Hylidae), revealed by microdissection, chromosome painting, and reverse hybridisationen
dc.typeArtigo
dcterms.rightsHolderPensoft Publ
unesp.author.lattes0458077399058762[5]
unesp.author.lattes8422327495725206
unesp.author.orcid0000-0002-7044-5764[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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