Is there post-meiotic transcriptional activity during hemipteran spermiogenesis?

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dc.contributor.authorChaboli Alevi, Kaio Cesar [UNESP]
dc.contributor.authorMendonaca, Priscila Pasqueetto [UNESP]
dc.contributor.authorPereira, Nathalia Paiva [UNESP]
dc.contributor.authorRosa, João Aristeu da [UNESP]
dc.contributor.authorVilela de Azeredo-Oliveira, Maria Tercilia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:54:33Z
dc.date.available2015-03-18T15:54:33Z
dc.date.issued2014-09-01
dc.description.abstractDifferent techniques have been used to evaluate post-meiotic nucleolar activity during spermatogenesis. However, the results have not been consistent, and transcriptional activity during spermiogenesis is still a highly debated issue. Through the use of cytogenetic tools, we evaluated whether the nucleolus is active during spermiogenesis in triatomines, more specifically, in Triatoma lenti. During spermatid differentiation, the nucleolus was observed using the cytogenetic technique of silver staining. Through the Feulgen reaction, we found that the silver ions had labeled the ribosomal DNA (rDNA). Using molecular cytogenetic techniques (DAPI and FISH), we were able to highlight the nucleolus and the Nucleolus Organizing Region (NOR) in spermatid images. Using the C-banding technique, we found rDNA and the NOR to be completely heterochromatic. Since this nuclear structure is present throughout the spermatid stage, we believe that the rDNA and NOR are inactivated by epigenetic factors, because we found a direct relationship between the nucleolus, rDNA, the NOR, and heterochromatin. Because heterochromatization of DNA is an important way to inactivate genetic material, we believe that the entire transcriptional activity required for cellular differentiation during spermiogenesis is mediated by the chromatoid body.en
dc.description.affiliationSao Paulo State Univ Julio de Mesquita Filho UNE, Inst Biosci Humanities & Exact Sci, Dept Biol, Cell Biol Lab, BR-15054000 Sao Paulo, Brazil
dc.description.affiliationSao Paulo State Univ Julio de Mesquita Filho, UNESP FCFAR, Coll Pharmaceut Sci, Dept Biol Sci,Lab Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ Julio de Mesquita Filho UNE, Inst Biosci Humanities & Exact Sci, Dept Biol, Cell Biol Lab, BR-15054000 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ Julio de Mesquita Filho, UNESP FCFAR, Coll Pharmaceut Sci, Dept Biol Sci,Lab Parasitol, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent193-198
dc.identifierhttp://dx.doi.org/10.1080/07924259.2014.889767
dc.identifier.citationInvertebrate Reproduction & Development. Abingdon: Taylor & Francis Ltd, v. 58, n. 3, p. 193-198, 2014.
dc.identifier.doi10.1080/07924259.2014.889767
dc.identifier.issn0792-4259
dc.identifier.urihttp://hdl.handle.net/11449/116947
dc.identifier.wosWOS:000338105700005
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofInvertebrate Reproduction & Development
dc.relation.ispartofjcr1.056
dc.relation.ispartofsjr0,409
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcytogeneticsen
dc.subjectnucleolusen
dc.subjectepigeneticsen
dc.subjectchromatoid bodyen
dc.titleIs there post-meiotic transcriptional activity during hemipteran spermiogenesis?en
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Ltd
unesp.author.orcid0000-0003-2111-638X[5]
unesp.author.orcid0000-0001-8333-3764[1]
unesp.author.orcid0000-0001-6318-3025[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Biologia - IBILCE
Artigos - Ciências Biológicas - FCFAR