Development and validation of an assay for measurement of leptin in pig saliva

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dc.contributor.authorSchmidt, Elizabeth M.S. [UNESP]
dc.contributor.authorEscribano, Damián
dc.contributor.authorMartinez-Subiela, Silvia
dc.contributor.authorMartinez-Miró, Silvia
dc.contributor.authorHernández, Fuensanta
dc.contributor.authorTvarijonaviciute, Asta
dc.contributor.authorCerón, José J.
dc.contributor.authorTecles, Fernando
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionVeterinary Clinical Hospital
dc.contributor.institutionVeterinary School
dc.date.accessioned2018-12-11T17:07:19Z
dc.date.available2018-12-11T17:07:19Z
dc.date.issued2016-10-28
dc.description.abstractBackground: Leptin has been measured in human in saliva samples. However, the low leptin concentration found in this biological fluid makes necessary the use of high sensitive methods. To the authors' knowledge, leptin has not been measured in porcine saliva. This study aimed to develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for salivary leptin measurements in pigs, using a species-specific antibody, and to evaluate how salivary leptin changes with body weight, food ingestion, and in experimental models of stress and inflammation. Polyclonal antibodies were produced in rabbits immunized with recombinant porcine leptin and used to develop a sandwich TR-IFMA. Results: The method had intra-assay and inter-assay coefficients of variation lower than 10 and 16 %, respectively. The assay was accurate and the low limit of detection allowed detection of leptin in all analyzed samples. Salivary leptin concentration was positively correlated to body weight (r = 0.58, P = 0.01) and increased after food ingestion (P < 0.001) and after 24 h of applying a model of experimental inflammation by turpentine injection (P < 0.05). However, it did not significantly change after a model of acute stress consisting of a nose snare restraining. Conclusion: These results indicate that the developed assay can measure leptin in porcine saliva in a reliable way and that leptin in saliva is influenced by body weight, food ingestion and inflammation.en
dc.description.affiliationSão Paulo State University (FMVZ - UNESP) Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science, campus of Botucatu. Rua Dr. Walter Mauricio Correa, s/n
dc.description.affiliationUniversity of Murcia Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU) Veterinary Clinical Hospital, Campus of Excellence Mare Nostrum
dc.description.affiliationUniversity of Murcia Animal Production Department Veterinary School, Campus of Excellence Mare Nostrum
dc.description.affiliationUniversity Autonoma of Barcelona Department of Animal Medicine and Surgery Veterinary School
dc.description.affiliationUnespSão Paulo State University (FMVZ - UNESP) Department of Veterinary Clinical Sciences School of Veterinary Medicine and Animal Science, campus of Botucatu. Rua Dr. Walter Mauricio Correa, s/n
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: PDE 203241/2014-2
dc.identifierhttp://dx.doi.org/10.1186/s12917-016-0871-9
dc.identifier.citationBMC Veterinary Research, v. 12, n. 1, 2016.
dc.identifier.doi10.1186/s12917-016-0871-9
dc.identifier.file2-s2.0-84992609385.pdf
dc.identifier.issn1746-6148
dc.identifier.scopus2-s2.0-84992609385
dc.identifier.urihttp://hdl.handle.net/11449/173694
dc.language.isoeng
dc.relation.ispartofBMC Veterinary Research
dc.relation.ispartofsjr0,934
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectInflammation
dc.subjectLeptin
dc.subjectPorcine saliva
dc.subjectStress
dc.subjectValidation
dc.titleDevelopment and validation of an assay for measurement of leptin in pig salivaen
dc.typeArtigo

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