Pathogenicity and Competitive Fitness of Salmonella enterica Serovar 4,[5],12:i:- Compared to Salmonella Typhimurium and Salmonella Derby in Swine

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dc.contributor.authorNaberhaus, Samantha A.
dc.contributor.authorKrull, Adam C.
dc.contributor.authorArruda, Bailey L.
dc.contributor.authorArruda, Paulo
dc.contributor.authorSahin, Orhan
dc.contributor.authorSchwartz, Kent J.
dc.contributor.authorBurrough, Eric R.
dc.contributor.authorMagstadt, Drew R.
dc.contributor.authorMatias Ferreyra, Franco
dc.contributor.authorGatto, Igor R. H. [UNESP]
dc.contributor.authorMeiroz de Souza Almeida, Henrique [UNESP]
dc.contributor.authorWang, Chong
dc.contributor.authorKreuder, Amanda J.
dc.contributor.institutionIowa State University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionVet Resources International - AMVC Management Services
dc.contributor.institutionCorteva Agriscience
dc.date.accessioned2020-12-12T01:15:17Z
dc.date.available2020-12-12T01:15:17Z
dc.date.issued2020-01-30
dc.description.abstractSince 2014, Salmonella 4,[5],12:i:- has emerged as the most common serovar of Salmonella enterica identified from swine samples submitted to veterinary diagnostic laboratories in the United States. To compare the pathogenicity of S. 4,[5],12:i:- in swine to the known pathogenic Salmonella Typhimurium and lesser pathogenic Salmonella Derby, 72 pigs (20 per Salmonella serovar treatment and 12 controls) were inoculated with either S. Typhimurium, S. 4,[5],12:i:-, S. Derby, or sham-inoculated and followed for up to 28 days thereafter via rectal temperature, fecal scoring, and fecal culture. Animals were euthanized on days 2, 4, or 28 to determine the gross and histopathologic signs of disease and tissue colonization. The results clearly demonstrate that for the isolates selected, serovar 4,[5],12:i:- possesses similar ability as serovar Typhimurium to cause clinical disease, colonize the tonsils and ileocecal lymph nodes, and be shed in the feces of infected swine past resolution of clinical disease. To compare the competitive fitness of S. 4,[5],12:i:- to S. Typhimurium in swine when co-infected, 12 pigs were co-inoculated with equal concentrations of both S. Typhimurium and S. 4,[5],12:i and followed for up to 10 days thereafter. When co-inoculated, serovar 4,[5],12:i:- was consistently detected in the feces of a higher percentage of pigs and at higher concentrations than serovar Typhimurium, suggesting an increased competitive fitness of 4,[5],12:i:- relative to serovar Typhimurium when inoculated simultaneously into naïve pigs. Whole genome sequencing analysis of the isolates used in these studies revealed similar virulence factor presence in all S. 4,[5],12:i:- and S. Typhimurium isolates, but not S. Derby, providing additional evidence for similar pathogenicity potential between serovars 4,[5],12:i:- and Typhimurium. Altogether, this data strongly supports the hypothesis that S. 4,[5],12:i:- is a pathogen of swine and suggests a mechanism through increased competitive fitness for the increasing identification of Salmonella 4,[5],12:i:- in swine diagnostic samples over the past several years.en
dc.description.affiliationDepartment of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine Iowa State University
dc.description.affiliationDepartment of Statistics College of Liberal Arts and Sciences Iowa State University
dc.description.affiliationDepartment of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine Iowa State University
dc.description.affiliationSchool of Agricultural and Veterinarian Sciences Preventive Veterinary Medicine and Animal Reproduction São Paulo State University (unesp)
dc.description.affiliationVet Resources International - AMVC Management Services
dc.description.affiliationCorteva Agriscience
dc.description.affiliationUnespSchool of Agricultural and Veterinarian Sciences Preventive Veterinary Medicine and Animal Reproduction São Paulo State University (unesp)
dc.description.sponsorshipNational Pork Board
dc.description.sponsorshipIdNational Pork Board: 16-215
dc.identifierhttp://dx.doi.org/10.3389/fvets.2019.00502
dc.identifier.citationFrontiers in Veterinary Science, v. 6.
dc.identifier.doi10.3389/fvets.2019.00502
dc.identifier.issn2297-1769
dc.identifier.scopus2-s2.0-85079481846
dc.identifier.urihttp://hdl.handle.net/11449/198525
dc.language.isoeng
dc.relation.ispartofFrontiers in Veterinary Science
dc.sourceScopus
dc.subject4,[5],12:i:-
dc.subjectDerby
dc.subjectmonophasic
dc.subjectpathogenesis
dc.subjectporcine
dc.subjectSalmonella
dc.subjectswine
dc.subjectTyphimurium
dc.titlePathogenicity and Competitive Fitness of Salmonella enterica Serovar 4,[5],12:i:- Compared to Salmonella Typhimurium and Salmonella Derby in Swineen
dc.typeArtigo

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