Diabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive rats

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dc.contributor.authorRosa, Camila Moreno [UNESP]
dc.contributor.authorXavier, Natasha Priscilla [UNESP]
dc.contributor.authorCampos, Dijon Henrique [UNESP]
dc.contributor.authorFernandes, Ana Angelica Henrique [UNESP]
dc.contributor.authorMariano Cezar, Marcelo Diarcadia [UNESP]
dc.contributor.authorMartinez, Paula Felippe [UNESP]
dc.contributor.authorCicogna, Antonio Carlos [UNESP]
dc.contributor.authorGimenes, Camila [UNESP]
dc.contributor.authorGimenes, Rodrigo [UNESP]
dc.contributor.authorOkoshi, Marina Politi [UNESP]
dc.contributor.authorOkoshi, Katashi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:30Z
dc.date.available2014-12-03T13:10:30Z
dc.date.issued2013-10-17
dc.description.abstractBackground: The combination of systemic arterial hypertension and diabetes mellitus (DM) induces greater cardiac remodeling than either condition alone. However, this association has been poorly addressed in senescent rats. Therefore, this study aimed to analyze the influence of streptozotocin-induced DM on ventricular remodeling and oxidative stress in aged spontaneously hypertensive rats (SHR).Methods: Fifty 18 month old male SHR were divided into two groups: control (SHR, n = 25) and diabetic (SHR-DM, n = 25). DM was induced by streptozotocin (40 mg/kg, i.p.). After nine weeks, the rats underwent echocardiography and myocardial functional study in left ventricular (LV) isolated papillary muscle preparations. LV samples were obtained to measure myocyte diameters, interstitial collagen fraction, and hydroxyproline concentration. Gene expression of atrial natriuretic peptide (ANP) and alpha- and beta-myosin heavy chain (MyHC) isoforms was evaluated by RT-PCR. Serum oxidative stress was assessed by measuring lipid hydroperoxide concentration and superoxide dismutase and glutathione peroxidase activities. Statistics: Student's t test or Mann-Whitney test, p < 0.05.Results: SHR-DM presented higher blood glucose (487 +/- 29 vs. 89.1 +/- 21.1 mg/dL) and lower body weight (277 +/- 26 vs. 339 +/- 38 g). Systolic blood pressure did not differ between groups. Echocardiography showed LV and left atrial dilation, LV diastolic and relative wall thickness decrease, and LV systolic and diastolic function impairment in SHR-DM. Papillary muscle study showed decreased myocardial contractility and contractile reserve in SHR-DM. Myocyte diameters and myocardial interstitial collagen fraction and hydroxyproline concentration did not differ between groups. Increased serum pro-oxidant activity and gene expression of ANP and beta/alpha-MyHC ratio were observed in DM.Conclusion: Diabetes mellitus induces cardiac dilation and functional impairment, increases oxidative stress and activates fetal gene program in aged spontaneously hypertensive rats.en
dc.description.affiliationSao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ, UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil
dc.description.affiliationUNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 306845/2012-1
dc.description.sponsorshipIdCNPq: 306857/2012-0
dc.description.sponsorshipIdFAPESP: 09/54506-7
dc.format.extent9
dc.identifierhttp://dx.doi.org/10.1186/1475-2840-12-152
dc.identifier.citationCardiovascular Diabetology. London: Biomed Central Ltd, v. 12, 9 p., 2013.
dc.identifier.doi10.1186/1475-2840-12-152
dc.identifier.fileWOS000327610800004.pdf
dc.identifier.issn1475-2840
dc.identifier.lattes9418970103564137
dc.identifier.lattes4463138671998432
dc.identifier.lattes1590971576309420
dc.identifier.urihttp://hdl.handle.net/11449/112201
dc.identifier.wosWOS:000327610800004
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofCardiovascular Diabetology
dc.relation.ispartofjcr5.235
dc.relation.ispartofsjr2,157
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDiabetes mellitusen
dc.subjectOxidative stressen
dc.subjectSystemic hypertensionen
dc.subjectSpontaneously hypertensive ratsen
dc.subjectElderlyen
dc.titleDiabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive ratsen
dc.typeArtigo
dcterms.rightsHolderBiomed Central Ltd
unesp.author.lattes9418970103564137[7]
unesp.author.lattes4463138671998432
unesp.author.lattes1590971576309420
unesp.author.orcid0000-0001-8980-8839[11]
unesp.author.orcid0000-0002-4402-6523[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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