Molecular and Serologic Detection of Ehrlichia spp. in Endangered Brazilian Wild Captive Felids

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dc.contributor.authorAndre, M. R. [UNESP]
dc.contributor.authorAdania, C. H.
dc.contributor.authorMachado, R. Z.
dc.contributor.authorAllegretti, S. M.
dc.contributor.authorFelippe, P. A. N.
dc.contributor.authorSilva, K. F. [UNESP]
dc.contributor.authorNakaghi, A. C. H. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAssoc Mata Ciliar
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T15:32:15Z
dc.date.available2014-05-20T15:32:15Z
dc.date.issued2010-07-01
dc.description.abstractEhrlichiosis, an emergent tickborne disease that affects both humans and animals, may represent a threat to the survival and preservation of wild felids in Brazil There are few studies of ehrlichiosis in wild felids in Brazil, but Ehrlichia spp are present in domestic cats Antibodies to Ehrlichia canis have been reported in a puma (Puma concolor) In this study we assessed the presence of these hemoparasites in the blood of Brazilian wild captive felids of the 72 animals tested, 5 (7%) were seropositive for the E cams antigen, and L1 (15%) were positive for E emirs DNA sequences We also performed sequence alignment to establish the identity of the parasite species infecting these animals using 16S rRNA and omp-1 genes Sequences based on 16S rRNA were similar to those found in dogs and cats from Thailand, Brazil, China, and Taiwan and with E canis obtained from a single individual (human) in Venezuela Ehrlichia sp sequence from sampled felines based on omp-1. gene was similar to the p28 and p30 multigene family of E canis To our knowledge, this is the first study of molecular detection of Ehrlichia sp in Brazilian wild feline speciesen
dc.description.affiliationUniv Estadual Paulista, Jaboticabal, SP, Brazil
dc.description.affiliationAssoc Mata Ciliar, Ctr Brasileiro Conservacao Felideos Neotropicais, Jundiai, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Jaboticabal, SP, Brazil
dc.format.extent1017-1023
dc.identifierhttp://www.jwildlifedis.org/content/46/3/1017.abstract
dc.identifier.citationJournal of Wildlife Diseases. Lawrence: Wildlife Disease Assoc, Inc, v. 46, n. 3, p. 1017-1023, 2010.
dc.identifier.issn0090-3558
dc.identifier.urihttp://hdl.handle.net/11449/41202
dc.identifier.wosWOS:000280690400043
dc.language.isoeng
dc.publisherWildlife Disease Assoc, Inc
dc.relation.ispartofJournal of Wildlife Diseases
dc.relation.ispartofjcr1.247
dc.relation.ispartofsjr0,760
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBrazilen
dc.subjectEhrlichia spen
dc.subjectPCRen
dc.subjectserologyen
dc.subjectwild felidsen
dc.titleMolecular and Serologic Detection of Ehrlichia spp. in Endangered Brazilian Wild Captive Felidsen
dc.typeArtigo
dcterms.licensehttp://wildlifedisease.org/wda/CONTACTUS/TERMS.aspx
dcterms.rightsHolderWildlife Disease Assoc, Inc
unesp.author.orcid0000-0003-4975-3663[4]
unesp.author.orcid0000-0002-1713-5222[1]

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