Eosinophilic inflammation in lymph nodes of dogs with visceral leishmaniasis

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dc.contributor.authorCosta, Sidnei Ferro [UNESP]
dc.contributor.authorTrivellato, Gabriel Franco [UNESP]
dc.contributor.authorRebech, Gabriela Torres [UNESP]
dc.contributor.authorOliveira dos Santos Maciel, Marilene [UNESP]
dc.contributor.authorMelo, Larissa Martins [UNESP]
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.authorde Lima, Valéria Marçal Felix [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:37:44Z
dc.date.available2018-12-11T17:37:44Z
dc.date.issued2018-08-01
dc.description.abstractEosinophils are traditionally associated with the immune response against helminth parasites. However, several studies have demonstrated that these cells have a role regarding protective immunity in leishmaniasis. Here, we examined the relationship between the presence of eosinophils and parasite load in biopsy samples from dogs, obtained through fine needle puncture and aspiration of lymph nodes. Histological slides containing biopsy material from lymph nodes of dogs with canine visceral leishmaniasis and healthy dogs were used to obtain baseline eosinophil counts. Subsequently, scrapings were taken from slides for DNA extraction and determination of parasite load, using real-time PCR (qRT-PCR). Additionally, production of nitric oxide (NO) and reactive oxygen species (ROS) levels by eosinophils in the peripheral blood of dogs with canine visceral leishmaniasis and healthy dogs was measured. The eosinophil percentage were higher in lymph nodes of infected group, and the parasite load showed a significant negative correlation with the eosinophil count. The production of NO and ROS by eosinophils in the peripheral blood was higher in the dogs with canine visceral leishmaniasis. All the results together suggest that eosinophils may participate in antileishmanial immunity in canine visceral leishmaniasis.en
dc.description.affiliationMaster's student of Animal Science School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationUndergraduate student of Animal Science School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationDoctoral student of Animal Science School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationDepartment of Surgery and Animal Reproduction School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationUnespMaster's student of Animal Science School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationUnespUndergraduate student of Animal Science School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationUnespDoctoral student of Animal Science School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Surgery and Animal Reproduction School of Veterinary Medicine São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 400063/2016-6
dc.identifierhttp://dx.doi.org/10.1111/pim.12567
dc.identifier.citationParasite Immunology, v. 40, n. 8, 2018.
dc.identifier.doi10.1111/pim.12567
dc.identifier.issn1365-3024
dc.identifier.issn0141-9838
dc.identifier.lattes4584674909952477
dc.identifier.scopus2-s2.0-85049866743
dc.identifier.urihttp://hdl.handle.net/11449/180028
dc.language.isoeng
dc.relation.ispartofParasite Immunology
dc.relation.ispartofsjr1,173
dc.relation.ispartofsjr1,173
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectdog
dc.subjecteosinophil
dc.subjectLeishmaniasis
dc.subjectlymph node
dc.subjectparasite load
dc.titleEosinophilic inflammation in lymph nodes of dogs with visceral leishmaniasisen
dc.typeArtigo
unesp.author.lattes4584674909952477
unesp.author.orcid0000-0002-0954-5045[7]

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA