Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions

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dc.contributor.authorPieri, Naira Caroline Godoy
dc.contributor.authorde Souza, Aline Fernanda
dc.contributor.authorBotigelli, Ramon Cesar [UNESP]
dc.contributor.authorPessôa, Laís Vicari de Figueiredo
dc.contributor.authorRecchia, Kaiana
dc.contributor.authorMachado, Lucas Simões
dc.contributor.authorGlória, Mayra Hirakawa
dc.contributor.authorde Castro, Raquel Vasconcelos Guimarães [UNESP]
dc.contributor.authorLeal, Diego Feitosa
dc.contributor.authorFantinato Neto, Paulo
dc.contributor.authorMartins, Simone Maria Massami Kitamura
dc.contributor.authordos Santos Martins, Daniele
dc.contributor.authorBressan, Fabiana Fernandes
dc.contributor.authorde Andrade, André Furugen Cesar
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSchool of Animal Sciences and Food Engineering
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T11:03:55Z
dc.date.available2021-06-25T11:03:55Z
dc.date.issued2021-01-01
dc.description.abstractCulture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells. Graphical abstract: [Figure not available: see fulltext.]en
dc.description.affiliationDepartment of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo
dc.description.affiliationDepartment of Veterinary Medicine School of Animal Sciences and Food Engineering
dc.description.affiliationDepartment of Pharmacology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Surgery Faculty of Veterinary Medicine and Animal Sciences University of Sao Paulo
dc.description.affiliationDepartment of Biochemistry Paulista School of Medicine Federal University of São Paulo (UNIFESP)
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Animal Science Faculty of Animal Science and Food Engineering University of São Paulo
dc.description.affiliationUnespDepartment of Pharmacology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1007/s12015-021-10198-8
dc.identifier.citationStem Cell Reviews and Reports.
dc.identifier.doi10.1007/s12015-021-10198-8
dc.identifier.issn2629-3277
dc.identifier.issn2629-3269
dc.identifier.scopus2-s2.0-85107761926
dc.identifier.urihttp://hdl.handle.net/11449/207956
dc.language.isoeng
dc.relation.ispartofStem Cell Reviews and Reports
dc.sourceScopus
dc.subjectPGCs
dc.subjectPorcine
dc.subjectRegenerative medicine
dc.subjectReproduction
dc.subjectTranslational medicine
dc.titlePorcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditionsen
dc.typeArtigo
unesp.author.orcid0000-0002-6800-6025[1]

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ