Anxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in mice

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dc.contributor.authorMiguel, Tarciso Tadeu [UNESP]
dc.contributor.authorNunes-de-Souza, Ricardo Luiz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2014-05-20T15:34:36Z
dc.date.available2014-05-20T15:34:36Z
dc.date.issued2011-08-01
dc.description.abstractChemical or electrical stimulation of the dorsal portion of the midbrain periaqueductal gray (dPAG) produces anxiogenic and antinociceptive effects. In rats, chemical stimulation of dPAG by local infusion of the neuropeptide corticotropin-releasing factor (CRF) provokes anxiogenic effects in the elevated plus-maze test (EPM). CRF also produces antinociception when injected intracerebroventricularly in rats, however it remains unclear whether this response is also observed following CRF injection into the dPAG in mice. Yet, given that there are CRF1 and CRF2 receptor subtypes within the PAG, it is important to show in which receptor subtypes CRF exert its anxiogenic and antinociceptive effects in the dPAG. Here, we investigated the role of these receptors in the anxiogenic (assessed in the EPM) and antinociceptive (assessed by the Formalin test: 2.5% formalin injection into the right hind paw) effects following intra-dPAG infusion of CRF in mice. The results show that intra-dPAG injections of CRF (75 pmol/0.1 mu l and 150 pmol/0.2 mu l) produced dose-dependent anxiogenic and antinociceptive effects. In addition, local infusion of NBI 27914 (5-chloro-4-(N-(cyclopropyl) methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)-aminopyridine; 2 nmo1/0.2 pl), a CRF1 receptor antagonist, completely blocked both the anxiogenic and antinociceptive effects induced by local infusion of CRF, while that of antisauvagine 30 (ASV30; 1 nmol/0.2 mu l), a CRF2 receptor antagonist, did not alter the CRF effects. Present results are suggestive that CRF1 (but not CRF2) receptors play a crucial role in the anxiogenic and antinociceptive effects induced by CRF in the dPAG in mice. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniversidade Federal de São Carlos (UFSCar), Programa Interinst Posgrad Ciencias Fisiol, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Farmaceut, Farmacol Lab, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPrograma de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP (PADC)
dc.description.sponsorshipIdFAPESP: 05/05171-1
dc.description.sponsorshipIdCNPq: 303580/2009-7
dc.format.extent292-300
dc.identifierhttp://dx.doi.org/10.1016/j.yhbeh.2011.06.004
dc.identifier.citationHormones and Behavior. San Diego: Academic Press Inc. Elsevier B.V., v. 60, n. 3, p. 292-300, 2011.
dc.identifier.doi10.1016/j.yhbeh.2011.06.004
dc.identifier.fileWOS000293316000010.pdf
dc.identifier.issn0018-506X
dc.identifier.urihttp://hdl.handle.net/11449/42597
dc.identifier.wosWOS:000293316000010
dc.language.isoeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relation.ispartofHormones and Behavior
dc.relation.ispartofjcr4.418
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectAnxietyen
dc.subjectAntinociceptionen
dc.subjectCRF1 and CRF2 receptorsen
dc.subjectPeriaqueductal grayen
dc.subjectElevated plus-mazeen
dc.subjectMiceen
dc.titleAnxiogenic and antinociceptive effects induced by corticotropin-releasing factor (CRF) injections into the periaqueductal gray are modulated by CRF1 receptor in miceen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Inc. Elsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Princípios Ativos Naturais e Toxicologia - FCFAR