Azadirachtin-based insecticide impairs testis morphology and spermatogenesis of the southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae)

Página do item simplificado
dc.contributor.authorScudeler, Elton Luiz [UNESP]
dc.contributor.authorDaquila, Bruno Vinicius
dc.contributor.authorde Carvalho, Shelly Favorito [UNESP]
dc.contributor.authorConte, Helio
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authordos Santos, Daniela Carvalho [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionState University of Maringá
dc.date.accessioned2023-07-29T13:37:17Z
dc.date.available2023-07-29T13:37:17Z
dc.date.issued2023-05-01
dc.description.abstractBackground: In the search for alternative tools for integrated pest management, azadirachtin, a botanical insecticide, has been used with the most promising activity against Spodoptera spp., but the mechanism of cytotoxicity on reproductive organs remains unclear. Spodoptera eridania (Stoll, 1782) is a polyphagous pest with great economic importance that has become an important target to elucidate the action of azadirachtin on the reproductive organs of insect pests, helping to understand the deleterious effects caused by its exposure. This study evaluated the effects of chronic exposure to azadirachtin on the morphology and ultrastructure of S. eridania larval testes as well as larval development. Results: Azadirachtin exposure (6 or 18 mg a.i. L−1) caused a progressive increase in cumulative mortality and reduced gain in body mass after 5 days. Testicular structure indicated a reduction in their size with internal morphological changes such as spermatogonia, spermatogonial, spermatocytes and spermatid cysts in degeneration. The occurrence of cell death in germ and somatic cells was evidenced by the TUNEL technique. Electron microscopy revealed changes in cystic cells, such as cytoplasmic membrane rupture and cytoplasmic vacuolization. Chromatin compaction, changes in the rough endoplasmic reticulum and Golgi complex cisternae were observed in germ cells. Apoptotic bodies occurred between germ cell cysts. Conclusion: Azadirachtin damaged the testes of S. eridania larvae, and these changes compromised spermatogenesis and consequently the development of the reproductive potential of this specimen, making azadirachtin a promising botanical insecticide for application in integrated pest management programs. © 2022 Society of Chemical Industry.en
dc.description.affiliationLaboratory of Insects Department of Structural and Functional Biology Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationLaboratory of Biological Control and Bioprospection of Insects. Department of Biotechnology Genetics and Cell Biology State University of Maringá, Maringá
dc.description.affiliationElectron Microscopy Center Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationDepartment of Biodiversity and Biostatistics Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationUnespLaboratory of Insects Department of Structural and Functional Biology Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationUnespElectron Microscopy Center Institute of Biosciences São Paulo State University, Botucatu
dc.description.affiliationUnespDepartment of Biodiversity and Biostatistics Institute of Biosciences São Paulo State University, Botucatu
dc.format.extent1650-1659
dc.identifierhttp://dx.doi.org/10.1002/ps.7338
dc.identifier.citationPest Management Science, v. 79, n. 5, p. 1650-1659, 2023.
dc.identifier.doi10.1002/ps.7338
dc.identifier.issn1526-4998
dc.identifier.issn1526-498X
dc.identifier.scopus2-s2.0-85146328700
dc.identifier.urihttp://hdl.handle.net/11449/248201
dc.language.isoeng
dc.relation.ispartofPest Management Science
dc.sourceScopus
dc.subjectbiopesticide
dc.subjectcytotoxicity
dc.subjecthistopathology
dc.subjectpest control
dc.subjectultrastructure
dc.titleAzadirachtin-based insecticide impairs testis morphology and spermatogenesis of the southern armyworm Spodoptera eridania (Lepidoptera: Noctuidae)en
dc.typeArtigo
unesp.author.orcid0000-0002-6005-1139[1]
unesp.author.orcid0000-0003-3540-3187[2]
unesp.author.orcid0000-0001-8062-104X[6]

Arquivos

Coleções

Rio Claro - CEIS - Centro de Estudos de Insetos Sociais
Artigos - Bioestatística - IBB
Botucatu - CME - Centro de Microscopia Eletrônica