Sugar production from wheat straw biomass by alkaline extrusion and enzymatic hydrolysis

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dc.contributor.authorCoimbra, Michelle Cardoso [UNESP]
dc.contributor.authorDuque, Aleta
dc.contributor.authorSaez, Felicia
dc.contributor.authorManzanares, Paloma
dc.contributor.authorGarcia-Cruz, Crispin Humberto [UNESP]
dc.contributor.authorBallesteros, Mercedes
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCIEMAT
dc.date.accessioned2018-11-26T16:17:22Z
dc.date.available2018-11-26T16:17:22Z
dc.date.issued2016-02-01
dc.description.abstractOne characteristic necessary to make ethanol production from biomass economically feasible is to optimize enzymatic dosage, since enzymes production is expensive. This work investigated the efficacy of different enzymes dosages and solid loadings on wheat straw enzymatic hydrolysis, aimed at obtaining process conditions that lead to good sugars yields from pretreated material. Alkaline extrusion was employed as pretreatment at 70 degrees C and 10% NaOH solution (w/v). Enzymatic hydrolysis was performed at 5, 10, 15 and 20% solids loading (w/v). Enzyme doses ranged from 6.92 to 20 FPU/g of glucan. Cellulase was also supplemented with xylanase at various proportions. Alkaline extrusion provided a substrate easier to hydrolyze than untreated material. Even the assay with the lowest enzyme dosage (6.92 FPU) achieved a good carbohydrate hydrolysis yield in relation to the theoretical; the glucose yield was 73.8% and xylose yield was 82.8%. A medium containing 100 g/L of fermentable sugar was obtained at 20% solids loading (w/v) and 20 FPU/g of glucan. The supplementation of cellulase with xylanase at U to FPU activity ratio of 3.11:1 improved the glucose yield about 21% over the assay with no xylanase. (C) 2015 Elsevier Ltd. All rights reserved.en
dc.description.affiliationSao Paulo State Univ, Dept Food Engn & Technol, BR-15054000 Sao Jose Do Rio Preto, Brazil
dc.description.affiliationCIEMAT, Dept Energy, Biofuels Unit, E-28040 Madrid, Spain
dc.description.affiliationUnespSao Paulo State Univ, Dept Food Engn & Technol, BR-15054000 Sao Jose Do Rio Preto, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipRESTOENE-2 project - Consejeria de Educacion, Comunidad de Madrid, Spain
dc.description.sponsorshipIdFAPESP: 2013/23525-1
dc.description.sponsorshipIdRESTOENE-2 project - Consejeria de Educacion, Comunidad de Madrid, Spain: P2013/MAE-2882
dc.format.extent1060-1068
dc.identifierhttp://dx.doi.org/10.1016/j.renene.2015.09.026
dc.identifier.citationRenewable Energy. Oxford: Pergamon-elsevier Science Ltd, v. 86, p. 1060-1068, 2016.
dc.identifier.doi10.1016/j.renene.2015.09.026
dc.identifier.fileWOS000364248300103.pdf
dc.identifier.issn0960-1481
dc.identifier.lattes3761109299906668
dc.identifier.urihttp://hdl.handle.net/11449/160947
dc.identifier.wosWOS:000364248300103
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRenewable Energy
dc.relation.ispartofsjr1,847
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCellulase supplementation
dc.subjectEnzymatic hydrolysis
dc.subjectEthanol
dc.subjectExtrusion
dc.subjectLignocellulose
dc.subjectXylanase
dc.titleSugar production from wheat straw biomass by alkaline extrusion and enzymatic hydrolysisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3761109299906668[5]
unesp.author.orcid0000-0003-4326-2185[6]

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Artigos - Engenharia e Tecnologia de Alimentos - IBILCE