Cold atmospheric plasma jet as a possible adjuvant therapy for periodontal disease

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dc.contributor.authorKoga-ito, Cristiane Yumi [UNESP]
dc.contributor.authorLima, Gabriela de Morais Gouvêa [UNESP]
dc.contributor.authorBorges, Aline Chiodi [UNESP]
dc.contributor.authorNishime, Thalita Mayumi Castaldelli
dc.contributor.authorSantana-Melo, Gabriela de Fatima [UNESP]
dc.contributor.authorKostov, Konstantin Georgiev
dc.contributor.authorMayer, Marcia Pinto Alves
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2022-01-20T17:47:08Z
dc.date.available2022-01-20T17:47:08Z
dc.date.issued2021-09-15
dc.description.abstract: Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once—Group P1 (n = 10); twice (Day 11 and 13)—Group P2 (n = 10); or not treated—Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications.en
dc.description.affiliationOral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos
dc.description.affiliationDepartment of Environment Engineering, São José dos Campos Institute of Science & Technology, São Paulo State University (UNESP), São José dos Campos
dc.description.affiliationLeibniz Institute for Plasma Science and Technology
dc.description.affiliationDepartment of Physics, Faculty of Engineering in Guaratinguetá, São Paulo State University (UNESP), Guaratinguetá
dc.description.affiliationDepartment of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo (USP), São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 15/03694-3
dc.description.sponsorshipIdFAPESP: 16/07196-6
dc.identifier.citationLIMA, G. M. G.; BORGES, A. C.; NISHIME, T. M. C.; SANTANA-MELO, G. F.; KOSTOV, K. G.; MAYER, M. P. A; KOGA-ITO, C. Y. Cold atmospheric plasma jet as a possible adjuvant therapy for periodontal disease. Molecules, v. 26, n. 18, p. 5590, 2021.pt
dc.identifier.issn1420-3049
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.urihttp://hdl.handle.net/11449/215985
dc.language.isoeng
dc.publisherMDPI
dc.relationhttps://doi.org/10.3390/molecules26185590pt
dc.relation.ispartofMoleculespt
dc.rights.accessRightsAcesso aberto
dc.subjectBiocompatibilityen
dc.subjectCold plasmaen
dc.subjectDisinfectionen
dc.subjectPeriodontitisen
dc.subjectPorphyromonas gingivalisen
dc.titleCold atmospheric plasma jet as a possible adjuvant therapy for periodontal diseaseen
dc.typeArtigo
unesp.author.lattes6543563161403421[1]
unesp.author.orcid0000-0002-2416-2173[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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Artigos - Engenharia Ambiental - São José dos Campos