Production of extracellular acid proteases by Aspergillus clavatus

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dc.contributor.authorTremacoldi, C. R.
dc.contributor.authorWatanabe, N. K.
dc.contributor.authorCarmona, E. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:56:04Z
dc.date.available2014-05-20T13:56:04Z
dc.date.issued2004-08-01
dc.description.abstractThe production of extracellular acid proteases from Aspergillus clavatus was evaluated in a culture filtrate medium, with different carbon and nitrogen sources. The fungus was cultivated at three different temperatures during 10 days. The proteolytic activity was determined on haemoglobin pH 5.0 at 37 degreesC. The highest acid proteolytic activity (80 U/ml) was observed in culture medium containing glucose and gelatin at 1% (w/v) at 30 degreesC at the third day of incubation. Cultures developed in Vogel medium with glucose at 2% (w/v) showed at about 45% of proteolytic activity when compared to the cultures with 1% of the same sugar. The optimum pH of enzymatic activity was 2.0 and the enzyme was stable at pH values ranging from 2.0 to 4.0. The optimum temperature was 40 degreesC and the half-lives at 40, 45 and 50 degreesC were 30, 10 and 5 min, respectively.en
dc.description.affiliationUniv Estadual Paulista Julio de Mesquita Filho, Dept Biochem & Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio de Mesquita Filho, Dept Biochem & Microbiol, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent639-642
dc.identifierhttp://dx.doi.org/10.1023/B:WIBI.0000043194.21080.c1
dc.identifier.citationWorld Journal of Microbiology & Biotechnology. Dordrecht: Kluwer Academic Publ, v. 20, n. 6, p. 639-642, 2004.
dc.identifier.doi10.1023/B:WIBI.0000043194.21080.c1
dc.identifier.issn0959-3993
dc.identifier.lattes4110421764783871
dc.identifier.urihttp://hdl.handle.net/11449/20053
dc.identifier.wosWOS:000225403300019
dc.language.isoeng
dc.publisherKluwer Academic Publ
dc.relation.ispartofWorld Journal of Microbiology & Biotechnology
dc.relation.ispartofjcr2.100
dc.relation.ispartofsjr0,604
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectacid proteasept
dc.subjectAspergillus clavatuspt
dc.subjectenzyme characterizationpt
dc.subjectoptimizationpt
dc.subjectproductionpt
dc.titleProduction of extracellular acid proteases by Aspergillus clavatusen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderKluwer Academic Publ
unesp.author.lattes4110421764783871
unesp.author.orcid0000-0002-0230-5735[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Bioquímica e Microbiologia - IBRC