Histomorphometry evaluation and cellular proliferation in superficial corneal ulcer, induced in rabbits, after using eyedrops of Citrus lemon

Página do item simplificado
dc.contributor.authorPerches, C. S. [UNESP]
dc.contributor.authorPellizzon, C. H. [UNESP]
dc.contributor.authorRanzani, J. J. T. [UNESP]
dc.contributor.authorPadovani, C. R. [UNESP]
dc.contributor.authorMerlini, N. B. [UNESP]
dc.contributor.authorSereno, M. G.
dc.contributor.authorFonzar, J. F. [UNESP]
dc.contributor.authorBrandao, C. V. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-27T06:00:15Z
dc.date.available2018-11-27T06:00:15Z
dc.date.issued2015-12-01
dc.description.abstractThe aim of this study was to evaluate and compare through histomorphometry and immunohistochemistry for PCNA (Proliferating Cell Nuclear Antigen), the repair process in superficial corneal ulcers induced in rabbits and treated with eyedrops of Citrus lemon (CL) essential oil. Fifty female rabbits were used and divided into 4 experimental groups of 10 animals each one. Every animal underwent induction of experimental superficial ulcer by topical application of n-heptanol. Three groups were treated with eyedrops of Citrus lemon essential oil in two different concentrations: 3% (GL3) and 5% (GL5). Another group was treated with Tween 80 8% (GT), which is the solvent used in the production of eyedrops of CL; the control group (CG) received only tear substitute. All eyedrops were applied four times daily. The experimental groups were divided into two subgroups with five animals in each one, according to the final evaluation periods. The first subgroup (M1) was evaluated after 24 hours and the second (M2) after 5 days. In the comparison between the initial and final moments, the groups treated with tear substitute, Tween 80 8% and eyedrops of Citrus lemon essential oil 5% had an increase in epithelial thickness at the periphery of the cornea and a higher percentage of cell proliferation. There was no difference in cellularity between treatments. The eyedrops of Citrus lemon essential oil, at different concentrations, promoted corneal reepithelialization without causing further injury to the epithelium and corneal stroma, so they can be used on the ocular surface.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Botucatu, SP, Brazil
dc.format.extent1660-1668
dc.identifierhttp://dx.doi.org/10.1590/1678-4162-7963
dc.identifier.citationArquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 67, n. 6, p. 1660-1668, 2015.
dc.identifier.doi10.1590/1678-4162-7963
dc.identifier.fileS0102-09352015000601660.pdf
dc.identifier.issn0102-0935
dc.identifier.lattes0019393779801069
dc.identifier.lattes1087615389013655
dc.identifier.orcid0000-0002-4494-4180
dc.identifier.orcid0000-0002-2011-5214
dc.identifier.scieloS0102-09352015000601660
dc.identifier.urihttp://hdl.handle.net/11449/165013
dc.identifier.wosWOS:000367094600024
dc.language.isopor
dc.publisherArquivo Brasileiro Medicina Veterinaria Zootecnia
dc.relation.ispartofArquivo Brasileiro De Medicina Veterinaria E Zootecnia
dc.relation.ispartofsjr0,248
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectsicilian lemon
dc.subjectcornea
dc.subjectulcer
dc.subjectPCNA
dc.subjecthistology
dc.titleHistomorphometry evaluation and cellular proliferation in superficial corneal ulcer, induced in rabbits, after using eyedrops of Citrus lemonen
dc.typeArtigo
dcterms.rightsHolderArquivo Brasileiro Medicina Veterinaria Zootecnia
unesp.author.lattes8727897080522289[4]
unesp.author.lattes0019393779801069[2]
unesp.author.lattes1087615389013655[8]
unesp.author.orcid0000-0002-7719-9682[4]
unesp.author.orcid0000-0002-4494-4180[2]
unesp.author.orcid0000-0002-2011-5214[8]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0102-09352015000601660.pdf
Tamanho:
404.58 KB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Cirurgia e Anestesiologia Veterinária - FMVZ
Artigos - Bioestatística - IBB