Genomic Features and Phylogenetic Analysis of Antimicrobial-Resistant Salmonella Mbandaka ST413 Strains

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dc.contributor.authorBenevides, Valdinete Pereira
dc.contributor.authorSaraiva, Mauro de Mesquita Souza
dc.contributor.authorNascimento, Camila de Fátima
dc.contributor.authorDelgado-Suárez, Enrique Jesús
dc.contributor.authorOliveira, Celso José Bruno
dc.contributor.authorSilva, Saura Rodrigues
dc.contributor.authorMiranda, Vitor Fernandes Oliveira
dc.contributor.authorChristensen, Henrik
dc.contributor.authorOlsen, John Elmerdahl
dc.contributor.authorBerchieri Junior, Angelo
dc.date.accessioned2024-03-04T13:09:27Z
dc.date.available2024-03-04T13:09:27Z
dc.date.issued2024-02-01
dc.description.abstractIn recent years, Salmonella enterica subsp. enterica serovar Mbandaka (S. Mbandaka) has been increasingly isolated from laying hens and shell eggs around the world. Moreover, this serovar has been identified as the causative agent of several salmonellosis outbreaks in humans. Surprisingly, little is known about the characteristics of this emerging serovar, and therefore, we investigated antimicrobial resistance, virulence, and prophage genes of six selected Brazilian strains of Salmonella Mbandaka using Whole Genome Sequencing (WGS). Multi-locus sequence typing revealed that the tested strains belong to Sequence Type 413 (ST413), which has been linked to recent multi-country salmonellosis outbreaks in Europe. A total of nine resistance genes were detected, and the most frequent ones were aac(6′)-Iaa, sul1, qacE, blaOXA-129, tet(B), and aadA1. A point mutation in ParC at the 57th position (threonine → serine) associated with quinolone resistance was present in all investigated genomes. A 112,960 bp IncHI2A plasmid was mapped in 4/6 strains. This plasmid harboured tetracycline (tetACDR) and mercury (mer) resistance genes, genes contributing to conjugative transfer, and genes involved in plasmid maintenance. Most strains (four/six) carried Salmonella genomic island 1 (SGI1). All S. Mbandaka genomes carried seven pathogenicity islands (SPIs) involved in intracellular survival and virulence: SPIs 1-5, 9, and C63PI. The virulence genes csgC, fimY, tcfA, sscA, (two/six), and ssaS (one/six) were absent in some of the genomes; conversely, fimA, prgH, and mgtC were present in all of them. Five Salmonella bacteriophage sequences (with homology to Escherichia phage phiV10, Enterobacteria phage Fels-2, Enterobacteria phage HK542, Enterobacteria phage ST64T, Salmonella phage SW9) were identified, with protein counts between 31 and 54, genome lengths of 24.7 bp and 47.7 bp, and average GC content of 51.25%. In the phylogenetic analysis, the genomes of strains isolated from poultry in Brazil clustered into well-supported clades with a heterogeneous distribution, primarily associated with strains isolated from humans and food. The phylogenetic relationship of Brazilian S. Mbandaka suggests the presence of strains with high epidemiological significance and the potential to be linked to foodborne outbreaks. Overall, our results show that isolated strains of S. Mbandaka are multidrug-resistant and encode a rather conserved virulence machinery, which is an epidemiological hallmark of Salmonella strains that have successfully disseminated both regionally and globally.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2020/07018-6
dc.description.sponsorshipIdFAPESP: 2021/10285-9
dc.description.sponsorshipIdCAPES: 001
dc.description.versionVersão final do editor
dc.formatPDF
dc.format.extent16 p.
dc.identifier.doihttps://doi.org/10.3390/microorganisms12020312
dc.identifier.issn2076-2607
dc.identifier.lattes8549597057103179
dc.identifier.lattes6849935103078317
dc.identifier.lattes7207595002088908
dc.identifier.lattes1085810832851989
dc.identifier.lattes3508096260678286
dc.identifier.orcid0000-0002-7552-1607
dc.identifier.orcid0000-0003-1875-4495
dc.identifier.orcid0000-0002-8639-4076
dc.identifier.orcid0000-0001-5380-8095
dc.identifier.orcid0000-0002-7761-0697
dc.identifier.orcid0000-0002-6333-5268
dc.identifier.orcid0000-0003-0574-9865
dc.identifier.orcid0000-0003-4238-4658
dc.identifier.orcid0000-0001-6225-6587
dc.identifier.orcid0000-0003-2522-6500
dc.identifier.urihttps://hdl.handle.net/11449/253509
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofMicroorganisms
dc.rights.accessRightsAcesso aberto
dc.subjectSalmonelosept
dc.subjectFatores de virulênciapt
dc.subjectBacteriófagospt
dc.subjectPatogênesept
dc.titleGenomic Features and Phylogenetic Analysis of Antimicrobial-Resistant Salmonella Mbandaka ST413 Strains
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal

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Artigos - Patologia Veterinária - FCAV